验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, human colon tissue, human liver tissue, mouse liver tissue, mouse lung tissue |
| Positive IHC detected in | human liver tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16242-1-AP targets SDSL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag9114 Product name: Recombinant human SDSL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-167 aa of BC009849 Sequence: MDGPVAEHAKQEPFHVVTPLLESWALSQVAGMPVFLKCENVQPSGSFKIRGIGHFCQEMAKKGCRHLVCSSGGNAGIAAAYAARKLGIPATIVLPESTSLQVVQRLQGEGAEVQLTGKVWDEANLRAQELAKRDGWENVPPFDHPLIWKGHASLVQELKAVLRTPPG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serine dehydratase-like |
| 别名 | EC:4.3.1.17, EC:4.3.1.19, L serine deaminase, L threonine dehydratase, L-serine deaminase |
| 计算分子量 | 329 aa, 35 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC009849 |
| 基因名称 | SDSL |
| Gene ID (NCBI) | 113675 |
| RRID | AB_2254163 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96GA7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SDSL (full name: Serine Dehydratase-Like) is a pyridoxal phosphate (PLP)-dependent enzyme encoded by human SDSL gene, which is mainly located in cytoplasm. Biochemical activity: It is a multifunctional enzyme with weak L- serine ammonia-lyase and L- threonine dehydratase activities, and participates in the process of amino acid catabolism (catalyzing serine and threonine to be converted into pyruvate, α-ketobutyric acid and ammonia respectively). Studies have shown that compared with the homologous enzyme SDS in the liver, the activity of SDSL in human cancer cells is lower. Clinical and research significance: This protein plays a certain role in signal transduction and metabolic regulation. Abnormal expression or variation at the gene level is related to specific genetic diseases (such as some thyroid dysfunction) and cancer mechanism research. Homodimer can be formed.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SDSL antibody 16242-1-AP | Download protocol |
| IHC protocol for SDSL antibody 16242-1-AP | Download protocol |
| WB protocol for SDSL antibody 16242-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
