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验证数据展示

  • WB analysis of HepG2 using 16242-1-AP

    WB analysis of HepG2 using 16242-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16242-1-AP (SDSL antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16242-1-AP

    IHC staining of human liver using 16242-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SDSL antibody included
  • IHC staining of human liver using 16242-1-AP

    IHC staining of human liver using 16242-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SDSL antibody included
  • IHC staining of mouse liver using 16242-1-AP

    IHC staining of mouse liver using 16242-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SDSL antibody included
  • IHC staining of mouse liver using 16242-1-AP

    IHC staining of mouse liver using 16242-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16242-1-AP (SDSL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SDSL antibody included
  • IF Staining of HepG2 using 16242-1-AP

    IF Staining of HepG2 using 16242-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SDSL antibody (16242-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SDSL Polyclonal antibody

SDSL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16242-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

L-serine dehydratase/L-threonine deaminase, L-serine deaminase, L threonine dehydratase, L serine deaminase, EC:4.3.1.19

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, human colon tissue, human liver tissue, mouse liver tissue, mouse lung tissue
Positive IHC detected inhuman liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SDSL Ready-To-Use IHC Kit. SDSL primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16242-1-AP targets SDSL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SDSL fusion protein Ag9114 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serine dehydratase-like
别名 L-serine dehydratase/L-threonine deaminase, L-serine deaminase, L threonine dehydratase, L serine deaminase, EC:4.3.1.19
计算分子量 329 aa, 35 kDa
观测分子量 55 kDa
GenBank蛋白编号BC009849
基因名称 SDSL
Gene ID (NCBI) 113675
RRIDAB_2254163
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96GA7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SDSL antibody 16242-1-APDownload protocol
IHC protocol for SDSL antibody 16242-1-APDownload protocol
IF protocol for SDSL antibody 16242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols