• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SDS Polyclonal antibody

SDS Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16594-1-AP

Print datasheet

Synonyms

L serine deaminase, L threonine dehydratase, SDH, SDS, serine dehydratase, TDH

验证数据展示

  • WB analysis using 16594-1-AP

    WB analysis using 16594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16594-1-AP (SDS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16594-1-AP

    IHC staining of human liver using 16594-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16594-1-AP (SDS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 16594-1-AP

    IHC staining of human liver using 16594-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16594-1-AP (SDS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16594-1-AP targets SDS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen SDS fusion protein Ag9704 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine dehydratase
Synonyms L serine deaminase, L threonine dehydratase, SDH, SDS, serine dehydratase, TDH
Calculated Molecular Weight 218 aa, 23 kDa
Observed Molecular Weight35 kDa; 70 kDa
GenBank Accession NumberBC020750
Gene Symbol SDS
Gene ID (NCBI) 10993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20132
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

L-Serine dehydratase (SDS ; SDH) catalyzes the pyridoxal phosphate (PLP) dependent deamination of l-serine to yield pyruvate. In mammals, SDH is found predominantly in the liver. Extensive studies have been carried out on SDH from rat and the enzyme has been found to play an important role in gluconeogenesis; its activity is induced by the consumption of high-protein diets, starvation and other treatments. The enzyme exists as a dimer of identical subunits, with each subunit exhibiting a bilobal architecture. (PMID: 25380533, PMID: 21878319, PMID: 14646100)

实验方案

Product Specific Protocols
WB protocol for SDS antibody 16594-1-APDownload protocol
IHC protocol for SDS antibody 16594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols