SDHC Polyclonal antibody
SDHC Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse brain tissue, mouse liver tissue, rat liver tissue, mouse heart tissue, mouse kidney tissue, mouse lung tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
| IHC | See 1 publications below |
产品信息
14575-1-AP targets SDHC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rabbit |
| Immunogen | SDHC fusion protein Ag5775 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | succinate dehydrogenase complex, subunit C, integral membrane protein, 15kDa |
| Synonyms | CYB560, CYBL, PGL3, QPs 1, QPS1, SDH3, SDHC |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC033626 |
| Gene Symbol | SDHC |
| Gene ID (NCBI) | 6391 |
| RRID | AB_2183291 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99643 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SDHC(Succinate dehydrogenase cytochrome b560 subunit, mitochondrial) is also named as CYB560, SDH3 and belongs to the cytochrome b560 family. It is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHC are the cause of paragangliomas type 3 (PGL3) and paraganglioma and gastric stromal sarcoma (PGGSS). It has a transit peptide of 29 amino acid and has 5 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SDHC antibody 14575-1-AP | Download protocol |
| IHC protocol for SDHC antibody 14575-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. | ||
Nucleic Acids Res Base-excision repair deficiency alone or combined with increased oxidative stress does not increase mtDNA point mutations in mice. | ||
iScience Prevention and regression of megamitochondria and steatosis by blocking mitochondrial fusion in the liver. | ||
PLoS One Quantitative proteomics in A30P*A53T α-synuclein transgenic mice reveals upregulation of Sel1l. | ||
Cell Rep Mitochondrial SENP2 regulates the assembly of SDH complex under metabolic stress | ||
Front Immunol Comprehensive analysis of the prognosis, tumor microenvironment, and immunotherapy response of SDHs in colon adenocarcinoma |