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验证数据展示

  • WB analysis using 11840-1-AP (same clone as 11840-1-PBS)

    WB analysis using 11840-1-AP (same clone as 11840-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11840-1-AP (SDCCAG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11840-1-PBS in a different storage buffer formulation.

  • IP experiment of A549 using 11840-1-AP (same clone as 11840-1-PBS)

    IP experiment of A549 using 11840-1-AP (same clone as 11840-1-PBS)

    IP result of anti-SDCCAG1 (IP:11840-1-AP, 4ug; Detection:11840-1-AP 1:500) with A549 cells lysate 1800ug. This data was developed using the same antibody clone with 11840-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 11840-1-AP (same clone as 11840-1-PBS)

    IHC staining of human lung cancer using 11840-1-AP (same clone as 11840-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11840-1-AP (SDCCAG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11840-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 11840-1-AP (same clone as 11840-1-PBS)

    IHC staining of human lung cancer using 11840-1-AP (same clone as 11840-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11840-1-AP (SDCCAG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11840-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SDCCAG1 Polyclonal antibody, PBS Only

SDCCAG1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11840-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11840-1-PBS targets SDCCAG1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2467

Product name: Recombinant human SDCCAG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 48-397 aa of BC020794

Sequence: EDITLQSGRDELNEELIQEESSEDEGEYEEVRKDQDSVGEMKDEGEETLNYPDTTIDLSHLQPQRSIQKLASKEESSNSSDSKSQSRRHLSAKERREMKKKKLPSDSGDLEALEGKDKEKESTVHIETHQNTSKNVAAVQPMKRGQKSKMKKMKEKYKDQDEEDRELIMKLLGSAGSNKEEKGKKGKKGKTKDEPVKKQPQKPRGGQRVSDNIKKETPFLEVITHELQDFAVDDPHDDKEEQDLDQQGNEENLFDSLTGQPHPEDVLLFAIPICAPYTTMTNYKYKVKLTPGVQKKGKAAKTALNSFMHSKEATAREKDLFRSVKDTDLSRNIPGKVKVSAPNLLNVKRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serologically defined colon cancer antigen 1
别名
计算分子量 1076 aa, 123 kDa
观测分子量 130-140 kDa
GenBank蛋白编号BC020794
基因名称 SDCCAG1
Gene ID (NCBI) 9147
RRIDAB_2183413
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60524
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SDCCAG1, also named as NEMF, is a nuclear export mediator. It can be bound to translocon-engaged 60S subunits on native ER membranes. Human SDCCAG1 has been implicated in lung cancers and it may function as a tumor suppressor gene. SDCCAG1 inactivation contributes to the transformed state of human lung cancer cells (PMID:16103875, 10575219). NEMF is also regarded as a potential biomarker for the detection of active and latent TB infections (PMID: 26818387).

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