验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, MCF-7 cells, rat kidney tissue, Jurkat cells, mouse kidney tissue, L02 cells, SH-SY5Y cells, mouse lung tissue, rat lung tissue |
| Positive IP detected in | mouse lung tissue, A549 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-12 cells, MCF-7 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10886-1-AP targets Syndecan-3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Syndecan-3 fusion protein Ag1317 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | syndecan 3 |
| 别名 | SDC3, syndecan 3, SYND3, SDCN, N syndecan |
| 计算分子量 | 38 kDa |
| 观测分子量 | 60-70 kDa |
| GenBank蛋白编号 | BC013974 |
| 基因名称 | Syndecan-3 |
| Gene ID (NCBI) | 9672 |
| RRID | AB_2182999 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75056 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Syndecan-3 is a member o f the Syndecan proteoglycan family. It plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Syndecan-3 antibody 10886-1-AP | Download protocol |
| IHC protocol for Syndecan-3 antibody 10886-1-AP | Download protocol |
| IF protocol for Syndecan-3 antibody 10886-1-AP | Download protocol |
| IP protocol for Syndecan-3 antibody 10886-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Metabolic and chemical regulation of tRNA modification associated with taurine deficiency and human disease. | ||
Am J Pathol No haploinsufficiency but loss of heterozygosity for EXT in multiple osteochondromas. | ||
Am J Pathol Screening for potential targets for therapy in mesenchymal, clear cell, and dedifferentiated chondrosarcoma reveals Bcl-2 family members and TGFβ as potential targets. | ||
J Cell Sci Electrophoresis of cell membrane heparan sulfate regulates galvanotaxis in glial cells. | ||
BMC Cancer Prognostic significance of the expression of GFRα1, GFRα3 and syndecan-3, proteins binding ARTEMIN, in mammary carcinoma. | ||
Medicine (Baltimore) Two novel predictive biomarkers for osteosarcoma and glycolysis pathways: A profiling study on HS2ST1 and SDC3 |
