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验证数据展示

  • IHC staining of human kidney using 16773-1-AP (same clone as 16773-1-PBS)

    IHC staining of human kidney using 16773-1-AP (same clone as 16773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16773-1-AP (SCUBE3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16773-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 16773-1-AP (same clone as 16773-1-PBS)

    IHC staining of human kidney using 16773-1-AP (same clone as 16773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16773-1-AP (SCUBE3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16773-1-PBS in a different storage buffer formulation.

  • WB analysis using 16773-1-AP (same clone as 16773-1-PBS)

    WB analysis using 16773-1-AP (same clone as 16773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16773-1-AP (SCUBE3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16773-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SCUBE3 Polyclonal antibody, PBS Only

SCUBE3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16773-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

CEGF3, Signal peptide, CUB and EGF-like domain-containing protein 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16773-1-PBS targets SCUBE3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10085

Product name: Recombinant human SCUBE3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 643-992 aa of BC052263

Sequence: YYHGQTEQCVPCPAGTFQEREGQLSCDLCPGSDAHGPLGATNVTTCAGQCPPGQHSVDGFKPCQPCPRGTYQPEAGRTLCFPCGGGLTTKHEGAISFQDCDTKVQCSPGHYYNTSIHRCIRCAMGSYQPDFRQNFCSRCPGNTSTDFDGSTSVAQCKNRQCGGELGEFTGYIESPNYPGNYPAGVECIWNINPPPKRKILIVVPEIFLPSEDECGDVLVMRKNSSPSSITTYETCQTYERPIAFTARSRKLWINFKTSEANSARGFQIPYVTYDEDYEQLVEDIVRDGRLYASENHQEILKDKKLIKAFFEVLAHPQNYFKYTEKHKEMLPKSFIKLLRSKVSSFLRPYK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal peptide, CUB domain, EGF-like 3
别名 CEGF3, Signal peptide, CUB and EGF-like domain-containing protein 3
计算分子量 992 aa, 109 kDa
观测分子量 130 kDa
GenBank蛋白编号BC052263
基因名称 SCUBE3
Gene ID (NCBI) 222663
RRIDAB_2285430
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IX30
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SCUBE3 is a secreted glycoprotein that belongs to the SCUBE family. Members of the SCUBE family are secreted plasma membrane-associated proteins characterised by a N-terminal signal peptide sequence, multiple EGF domains, a N-linked glycosylated spacer region and a C-terminal CUB region. SCUBE3 may play a role in bone cell biology (PMID: 15234972). It may also has a role in the regulation of cardiac growth and remodeling responses (PMID: 17442284). Besides, SCUBE3 has been reported to be involved in lung cancer progression (PMID: 21441952; 23420440).

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