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SCRN3 Polyclonal antibody

SCRN3 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13240-1-AP

Print datasheet

Synonyms

SCRN3, secernin 3, SES3

验证数据展示

  • WB analysis of HepG2 using 13240-1-AP

    WB analysis of HepG2 using 13240-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13240-1-AP (SCRN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 13240-1-AP

    IP experiment of HEK-293 using 13240-1-AP

    IP result of anti-SCRN3 (IP:13240-1-AP, 4ug; Detection:13240-1-AP 1:800) with HEK-293 cells lysate 1760ug.

  • IHC staining of human pancreas using 13240-1-AP

    IHC staining of human pancreas using 13240-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 13240-1-AP

    IHC staining of human kidney using 13240-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 13240-1-AP

    IF Staining of HepG2 using 13240-1-AP

    Immunofluorescent analysis of HepG2 cells using 13240-1-AP (SCRN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, A549 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13240-1-AP targets SCRN3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen SCRN3 fusion protein Ag3777 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name secernin 3
Synonyms SCRN3, secernin 3, SES3
Calculated Molecular Weight 424 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC031821
Gene Symbol SCRN3
Gene ID (NCBI) 79634
RRIDAB_2184816
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ0VDG4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCRN genes encode protein members of the secernin family including Secernin 1, Secernin 2 and Secernin 3. Function of Secernin 2 and Secernin 3 are so far not well understood. Secernin 1 is a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin-1 a novel tumor-associated antigen (TAA) and may be a universal marker of different cancer types including gastric cancer, which has been validated by several researches. Measurement of SCRN1 can be used in the early detection of cancer or in the surveillance of patients who undergo surgery.

实验方案

Product Specific Protocols
WB protocol for SCRN3 antibody 13240-1-APDownload protocol
IHC protocol for SCRN3 antibody 13240-1-APDownload protocol
IF protocol for SCRN3 antibody 13240-1-APDownload protocol
IP protocol for SCRN3 antibody 13240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols