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验证数据展示

  • WB analysis of HepG2 using 13240-1-AP

    WB analysis of HepG2 using 13240-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13240-1-AP (SCRN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 13240-1-AP

    IP experiment of HEK-293 using 13240-1-AP

    IP result of anti-SCRN3 (IP:13240-1-AP, 4ug; Detection:13240-1-AP 1:800) with HEK-293 cells lysate 1760ug.

  • IHC staining of human pancreas using 13240-1-AP

    IHC staining of human pancreas using 13240-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 13240-1-AP

    IHC staining of human kidney using 13240-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 13240-1-AP

    IF Staining of HepG2 using 13240-1-AP

    Immunofluorescent analysis of HepG2 cells using 13240-1-AP (SCRN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 13240-1-AP

    IF Staining of HepG2 using 13240-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SCRN3 antibody (13240-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SCRN3 Polyclonal antibody

SCRN3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13240-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

SES3, Secernin-3, secernin 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, A549 cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13240-1-AP targets SCRN3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SCRN3 fusion protein Ag3777 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 secernin 3
别名 SES3, Secernin-3, secernin 3
计算分子量 424 aa, 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC031821
基因名称 SCRN3
Gene ID (NCBI) 79634
RRIDAB_2184816
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ0VDG4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCRN genes encode protein members of the secernin family including Secernin 1, Secernin 2 and Secernin 3. Function of Secernin 2 and Secernin 3 are so far not well understood. Secernin 1 is a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin-1 a novel tumor-associated antigen (TAA) and may be a universal marker of different cancer types including gastric cancer, which has been validated by several researches. Measurement of SCRN1 can be used in the early detection of cancer or in the surveillance of patients who undergo surgery.

实验方案

Product Specific Protocols
WB protocol for SCRN3 antibody 13240-1-APDownload protocol
IHC protocol for SCRN3 antibody 13240-1-APDownload protocol
IF protocol for SCRN3 antibody 13240-1-APDownload protocol
IP protocol for SCRN3 antibody 13240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols