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验证数据展示

  • WB analysis of BxPC-3 using 11485-2-AP

    WB analysis of BxPC-3 using 11485-2-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11485-2-AP (SCRN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 11485-2-AP

    IHC staining of human gliomas using 11485-2-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11485-2-AP (SCRN2 antibody) at dilution of 1:50 (under 40x lens).

SCRN2 Polyclonal antibody

SCRN2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11485-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SCRN2, secernin 2, Ses2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11485-2-AP targets SCRN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SCRN2 fusion protein Ag2001 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 secernin 2
别名 SCRN2, secernin 2, Ses2
计算分子量 46.6 kDa
观测分子量 40-47 kDa
GenBank蛋白编号BC017317
基因名称 SCRN2
Gene ID (NCBI) 90507
RRIDAB_2184813
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96FV2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCRN genes encode protein members of the secernin family including Secernin 1, Secernin 2 and Secernin 3. Function of Secernin 2 and Secernin 3 are so far not well understood. Secernin 1 is a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin-1 a novel tumor-associated antigen (TAA) and may be a universal marker of different cancer types including gastric cancer, which has been validated by several researches. Measurement of SCRN1 can be used in the early detection of cancer or in the surveillance of patients who undergo surgery.

实验方案

Product Specific Protocols
WB protocol for SCRN2 antibody 11485-2-APDownload protocol
IHC protocol for SCRN2 antibody 11485-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols