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验证数据展示

  • WB analysis using 12614-1-AP (same clone as 12614-1-PBS)

    WB analysis using 12614-1-AP (same clone as 12614-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12614-1-AP (SCO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 12614-1-AP (same clone as 12614-1-PBS)

    IP experiment of HepG2 using 12614-1-AP (same clone as 12614-1-PBS)

    IP result of anti-SCO1 (IP:12614-1-AP, 4ug; Detection:12614-1-AP 1:1000) with HepG2 cells lysate 1600 ug. This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 12614-1-AP (same clone as 12614-1-PBS)

    IHC staining of human stomach cancer using 12614-1-AP (same clone as 12614-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12614-1-AP (SCO1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12614-1-PBS in a different storage buffer formulation.

SCO1 Polyclonal antibody, PBS Only

SCO1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 12614-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

SCOD1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12614-1-PBS targets SCO1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3269

Product name: Recombinant human SCO1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-301 aa of BC015504

Sequence: MAMLVLVPGRVMRPLGGQLWRFLPRGLEFWGPAEGTARVLLRQFCARQAEAWRASGRPGYCLGTRPLSTARPPPPWSQKGPGDSTRPSKPGPVSWKSLAITFAIGGALLAGMKHVKKEKAEKLEKERQRHIGKPLLGGPFSLTTHTGERKTDKDYLGQWLLIYFGFTHCPDVCPEELEKMIQVVDEIDSITTLPDLTPLFISIDPERDTKEAIANYVKEFSPKLVGLTGTREEVDQVARAYRVYYSPGPKDEDEDYIVDHTIIMYLIGPDGEFLDYFGQNKRKGEIAASIATHMRPYRKKS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SCO cytochrome oxidase deficient homolog 1 (yeast)
别名 SCOD1
计算分子量 301 aa, 34 kDa
观测分子量 30 kDa
GenBank蛋白编号BC015504
基因名称 SCO1
Gene ID (NCBI) 6341
RRIDAB_2184641
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75880
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human SCO1 and SCO2 are metallochaperones that are essential for the assembly of the catalytic core of cytochrome c oxidase (COX). SCO1 appears to be involved in the folding and assembly of cytochrome oxidase subunit II, and possibly of other proteins of this subcompartment. It is a 33 kDa protein which displays limited homology to members of the DnaJ family(PMID:14731768). It is also named as SCOD1 and belongs to the SCO1/2 family. It can exsit as a homodimer(PMID:16735468). Defects in SCO1 are a cause of mitochondrial complex IV deficiency (MT-C4D).

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