验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue, PC-12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28079-1-AP targets SCN1A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag25942 Product name: Recombinant human SCN1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 456-522 aa of NM_001165963 Sequence: MEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sodium channel, voltage-gated, type I, alpha subunit |
| 别名 | sodium channel, Pan, FEB3, GEFSP2, HBSCI, NAC1 |
| 计算分子量 | 229 kDa |
| 观测分子量 | 229-240 kDa |
| GenBank蛋白编号 | NM_001165963 |
| 基因名称 | SCN1A |
| Gene ID (NCBI) | 6323 |
| RRID | AB_2881054 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35498 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sodium channel protein type 1 subunit alpha (SCN1A) is a multi-pass membrane protein that is crucial for generating and transmitting electrical signals in neurons, thereby contributing to the sensory perception of mechanically-induced pain (PMID: 14672992). SCN1A also regulates neuronal excitability and is essential for normal brain function (PMID: 37139072; 20831750).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SCN1A antibody 28079-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
