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验证数据展示

  • IF Staining of SH-SY5Y using 55334-1-AP

    IF Staining of SH-SY5Y using 55334-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 55334-1-AP (SCN10A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 55334-1-AP

    FC experiment of HeLa using 55334-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SCN10A (55334-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

SCN10A Polyclonal antibody

SCN10A Polyclonal Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 55334-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Voltage-gated sodium channel subunit alpha Nav1.8, SNS, PN3, Nav1.8, hPN3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55334-1-AP targets SCN10A in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sodium channel, voltage-gated, type X, alpha subunit
别名 Voltage-gated sodium channel subunit alpha Nav1.8, SNS, PN3, Nav1.8, hPN3
计算分子量 221 kDa
GenBank蛋白编号NM_006514
基因名称 SCN10A
Gene ID (NCBI) 6336
RRIDAB_2881309
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5Y9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for SCN10A antibody 55334-1-APDownload protocol
FC protocol for SCN10A antibody 55334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols