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SCN10A Polyclonal antibody

SCN10A Polyclonal Antibody for FC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 55334-1-AP

Print datasheet

Synonyms

hPN3, Nav1.8, PN3, SCN10A, SNS

验证数据展示

  • IF Staining of SH-SY5Y using 55334-1-AP

    IF Staining of SH-SY5Y using 55334-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 55334-1-AP (SCN10A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 55334-1-AP

    FC experiment of HeLa using 55334-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SCN10A (55334-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inSH-SY5Y cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55334-1-AP targets SCN10A in IF, FC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC, ELISA
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sodium channel, voltage-gated, type X, alpha subunit
Synonyms hPN3, Nav1.8, PN3, SCN10A, SNS
Calculated Molecular Weight 221 kDa
GenBank Accession NumberNM_006514
Gene Symbol SCN10A
Gene ID (NCBI) 6336
RRIDAB_2881309
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5Y9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for SCN10A antibody 55334-1-APDownload protocol
FC protocol for SCN10A antibody 55334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols