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验证数据展示

  • WB analysis using 25544-1-AP

    WB analysis using 25544-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25544-1-AP (SCML2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 25544-1-AP

    WB analysis of HEK-293 using 25544-1-AP

    WB result of SCML2 antibody (25544-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCML2 transfected HEK-293 cells.

  • IP experiment of HEK-293 using 25544-1-AP

    IP experiment of HEK-293 using 25544-1-AP

    IP result of anti-SCML2 (IP:25544-1-AP, 4ug; Detection:25544-1-AP 1:300) with HEK-293 cells lysate 2400ug.

  • IF Staining of HEK-293 using 25544-1-AP

    IF Staining of HEK-293 using 25544-1-AP

    Immunofluorescent analysis of HEK-293 cells using 25544-1-AP (SCML2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

SCML2 Polyclonal antibody

SCML2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 25544-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, ELISA

别名

SCML2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse testis tissue, K-562 cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25544-1-AP targets SCML2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag22271

Product name: Recombinant human SCML2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 563-649 aa of BC064617

Sequence: MYIHTSVSQDFSRSVPGTTSSPLVGDISPKSSPHEVKFQMQRKSEAPSYIAVPDPSVLKQGFSKDPSTWSVDEVIQFMKHTDPQISG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sex comb on midleg-like 2 (Drosophila)
别名 SCML2
计算分子量 700 aa, 77 kDa
观测分子量 70 kDa, 80 kDa
GenBank蛋白编号BC064617
基因名称 SCML2
Gene ID (NCBI) 10389
RRIDAB_2880127
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQR0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The human SCML2 gene, a mammalian homologue of the Drosophila PcG protein SCM, encodes two protein isoforms: SCML2A that is bound to chromatin and SCML2B that is predominantly nucleoplasmic (PMID: 24358021). Polycomb repressive complex-1 (PRC1) is essential for the epigenetic regulation of gene expression. SCML2 is a Polycomb-group protein that associates with PRC1. SCML2 participates in the epigenetic control of transcription directly and in cooperation with PRC1 (PMID: 24986859).

实验方案

Product Specific Protocols
WB protocol for SCML2 antibody 25544-1-APDownload protocol
IF protocol for SCML2 antibody 25544-1-APDownload protocol
IP protocol for SCML2 antibody 25544-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols