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SCML1 Polyclonal antibody

SCML1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15093-1-AP

Print datasheet

Synonyms

SCML1

验证数据展示

  • WB analysis using 15093-1-AP

    WB analysis using 15093-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 15093-1-AP (SCML1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 15093-1-AP

    IHC staining of mouse testis using 15093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15093-1-AP (SCML1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 15093-1-AP

    IHC staining of mouse testis using 15093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15093-1-AP (SCML1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 15093-1-AP

    IF Staining of HepG2 using 15093-1-AP

    Immunofluorescent analysis of HepG2 cells using 15093-1-AP (SCML1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15093-1-AP targets SCML1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen SCML1 fusion protein Ag5107 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sex comb on midleg-like 1 (Drosophila)
Synonyms SCML1
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 23 kDa，34kDa
GenBank Accession NumberBC026159
Gene Symbol SCML1
Gene ID (NCBI) 6322
RRIDAB_2153243
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UN30
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sex comb on midleg-like 1 (SCML1) is a meiosis-specific protein and is an essential component of the meiotic dense body. SCML1 is preferentially expressed in germ stem cells of testis, therefore likely involved in spermatogenesis.

实验方案

Product Specific Protocols
WB protocol for SCML1 antibody 15093-1-APDownload protocol
IHC protocol for SCML1 antibody 15093-1-APDownload protocol
IF protocol for SCML1 antibody 15093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols