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验证数据展示

  • WB analysis using 15093-1-AP

    WB analysis using 15093-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 15093-1-AP (SCML1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 15093-1-AP

    IHC staining of mouse testis using 15093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15093-1-AP (SCML1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 15093-1-AP

    IHC staining of mouse testis using 15093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15093-1-AP (SCML1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 15093-1-AP

    IF Staining of A549 using 15093-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SCML1 antibody (15093-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • FC experiment of A549 using 15093-1-AP

    FC experiment of A549 using 15093-1-AP

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SCML1 Polyclonal antibody (15093-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

SCML1 Polyclonal antibody

SCML1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 15093-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

Sex comb on midleg-like protein 1, SCML 1, Gm47, Gm1792

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15093-1-AP targets SCML1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 SCML1 fusion protein Ag5107 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sex comb on midleg-like 1 (Drosophila)
别名 Sex comb on midleg-like protein 1, SCML 1, Gm47, Gm1792
计算分子量 37 kDa
观测分子量30-35 kDa
GenBank蛋白编号BC026159
基因名称 SCML1
Gene ID (NCBI) 6322
RRIDAB_2153243
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UN30
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sex comb on midleg-like 1 (SCML1) is a meiosis-specific protein and is an essential component of the meiotic dense body. SCML1 is preferentially expressed in germ stem cells of testis, therefore likely involved in spermatogenesis.

实验方案

Product Specific Protocols
WB protocol for SCML1 antibody 15093-1-APDownload protocol
IHC protocol for SCML1 antibody 15093-1-APDownload protocol
IF protocol for SCML1 antibody 15093-1-APDownload protocol
FC protocol for SCML1 antibody 15093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols