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验证数据展示

  • WB analysis using 11489-1-AP

    WB analysis using 11489-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11489-1-AP (SCMH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11489-1-AP

    IHC staining of human pancreas cancer using 11489-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11489-1-AP (SCMH1 antibody) at dilution of 1:100 (under 10x lens).

SCMH1 Polyclonal antibody

SCMH1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11489-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Polycomb protein SCMH1, SCMH1, Scml3, Sex comb on midleg homolog 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-1080 cells, U-251 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11489-1-AP targets SCMH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SCMH1 fusion protein Ag1972 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sex comb on midleg homolog 1 (Drosophila)
别名 Polycomb protein SCMH1, SCMH1, Scml3, Sex comb on midleg homolog 1
计算分子量599aa,66 kDa; 660aa,73 kDa
观测分子量 58 kDa
GenBank蛋白编号BC021252
基因名称 SCMH1
Gene ID (NCBI) 22955
RRIDAB_2183702
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96GD3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SCMH1 antibody 11489-1-APDownload protocol
IHC protocol for SCMH1 antibody 11489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols