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验证数据展示

  • WB analysis using 11489-1-AP

    WB analysis using 11489-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11489-1-AP (SCMH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11489-1-AP

    IHC staining of human pancreas cancer using 11489-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11489-1-AP (SCMH1 antibody) at dilution of 1:100 (under 10x lens).

SCMH1 Polyclonal antibody

SCMH1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11489-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Polycomb protein SCMH1, SCMH1, Scml3, Sex comb on midleg homolog 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHT-1080 cells, U-251 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11489-1-AP targets SCMH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1972

Product name: Recombinant human SCMH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 290-599 aa of BC021252

Sequence: SSAMQAPTVCIYLNKNGSTGPHLDKKKVQQLPDHFGPARASVVLQQAVQACIDCAYHQKTVFSFLKQGHGGEVISAVFDREQHTLNLPAVNSITYVLRFLEKLCHNLRSDNLFGNQPFTQTHLSLTAIEYSHSHDRYLPGETFVLGNSLARSLEPHSDSMDSASNPTNLVSTSQRHRPLLSSCGLPPSTASAVRRLCSRGVLKGSNERRDMESFWKLNRSPGSDRYLESRDASRLSGRDPSSWTVEDVMQFVREADPQLGPHADLFRKHEIDGKALLLLRSDMMMKYMGLKLGPALKLSYHIDRLKQGKF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sex comb on midleg homolog 1 (Drosophila)
别名 Polycomb protein SCMH1, SCMH1, Scml3, Sex comb on midleg homolog 1
计算分子量599aa,66 kDa; 660aa,73 kDa
观测分子量 58 kDa
GenBank蛋白编号BC021252
基因名称 SCMH1
Gene ID (NCBI) 22955
RRIDAB_2183702
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96GD3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for SCMH1 antibody 11489-1-APDownload protocol
WB protocol for SCMH1 antibody 11489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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