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SCMH1 Polyclonal antibody

SCMH1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11489-1-AP

Print datasheet

Synonyms

Polycomb protein SCMH1, SCMH1, Scml3, Sex comb on midleg homolog 1

验证数据展示

  • WB analysis using 11489-1-AP

    WB analysis using 11489-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11489-1-AP (SCMH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11489-1-AP

    IHC staining of human pancreas cancer using 11489-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11489-1-AP (SCMH1 antibody) at dilution of 1:100 (under 10x lens).

经过测试的应用

Positive WB detected inHT-1080 cells, U-251 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11489-1-AP targets SCMH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen SCMH1 fusion protein Ag1972 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sex comb on midleg homolog 1 (Drosophila)
Synonyms Polycomb protein SCMH1, SCMH1, Scml3, Sex comb on midleg homolog 1
Calculated Molecular Weight599aa,66 kDa; 660aa,73 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC021252
Gene Symbol SCMH1
Gene ID (NCBI) 22955
RRIDAB_2183702
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96GD3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SCMH1 antibody 11489-1-APDownload protocol
IHC protocol for SCMH1 antibody 11489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols