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Secretogranin V Polyclonal antibody

Secretogranin V Polyclonal Antibody for IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IF/ICC, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10761-1-AP

Print datasheet

Synonyms

7B2, Neuroendocrine protein 7B2, P7B2, Pituitary polypeptide, SCG5, Secretogranin 5, Secretogranin V, secretogranin V (7B2 protein), SGNE1, SgV

验证数据展示

  • IHC staining of rat pancreas using 10761-1-AP

    IHC staining of rat pancreas using 10761-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat pancreas using 10761-1-AP

    IHC staining of rat pancreas using 10761-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10761-1-AP (Secretogranin V antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-12 using 10761-1-AP

    IF Staining of PC-12 using 10761-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-12 cells using 10761-1-AP (Secretogranin V antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inrat pancreas tissue, human pituitary adenoma tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

10761-1-AP targets Secretogranin V in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications IF/ICC, IHC, ELISA
Cited ApplicationsWB
Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse
Immunogen Secretogranin V fusion protein Ag1107 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name secretogranin V (7B2 protein)
Synonyms 7B2, Neuroendocrine protein 7B2, P7B2, Pituitary polypeptide, SCG5, Secretogranin 5, Secretogranin V, secretogranin V (7B2 protein), SGNE1, SgV
Calculated Molecular Weight 24 kDa
GenBank Accession NumberBC005349
Gene Symbol SCG5
Gene ID (NCBI) 6447
RRIDAB_2183280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05408
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Secretogranin V antibody 10761-1-APDownload protocol
IHC protocol for Secretogranin V antibody 10761-1-APDownload protocol
IF protocol for Secretogranin V antibody 10761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncotarget

Bromodomain protein BRD4 inhibitor JQ1 regulates potential prognostic molecules in advanced renal cell carcinoma.

Authors - Takashi Sakaguchi
View Article
mouseWB

Aging (Albany NY)

Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin

Authors - Yuqi Xin
View Article