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验证数据展示

  • WB analysis using 23844-1-AP (same clone as 23844-1-PBS)

    WB analysis using 23844-1-AP (same clone as 23844-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23844-1-AP (SCF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23844-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 23844-1-AP (same clone as 23844-1-PBS)

    IF Staining of HEK-293 using 23844-1-AP (same clone as 23844-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SCF antibody (23844-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23844-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 23844-1-AP (same clone as 23844-1-PBS)

    IF Staining of HepG2 using 23844-1-AP (same clone as 23844-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SCF antibody (23844-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23844-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 23844-1-AP (same clone as 23844-1-PBS)

    IHC staining of mouse kidney using 23844-1-AP (same clone as 23844-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23844-1-AP (SCF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23844-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 23844-1-AP (same clone as 23844-1-PBS)

    IHC staining of mouse kidney using 23844-1-AP (same clone as 23844-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23844-1-AP (SCF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23844-1-PBS in a different storage buffer formulation.

SCF Polyclonal antibody, PBS Only

SCF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 23844-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

KITLG, c Kit ligand, c-Kit ligand, KIT ligand, Kitl

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

23844-1-PBS targets SCF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag20837

Product name: Recombinant human SCF protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-273 aa of BC074725

Sequence: MKKTQTWILTCIYLQLLLFNPLVKTEGICRNRVTNNVKDVTKLVANLPKDYMITLKYVPGMDVLPSHCWISEMVVQLSDSLTDLLDKFSNISEGLSNYSIIDKLVNIVDDLVECVKENSSKDLKKSFKSPEPRLFTPEEFFRIFNRSIDAFKDFVVASETSDCVVSSTLSPEKDSRVSVTKPFMLPPVAASSLRNDSSSSNRKAKNPPGDSSLHWAAMALPALFSLIIGFAFGALYWKKRQPSLTRAVENIQINEEDNEISMLQEKEREFQEV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIT ligand
别名 KITLG, c Kit ligand, c-Kit ligand, KIT ligand, Kitl
计算分子量 273 aa, 31 kDa
观测分子量 31 kDa
GenBank蛋白编号BC074725
基因名称 SCF
Gene ID (NCBI) 4254
RRIDAB_3669419
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDP21583
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Stem cell factor (SCF) is also named as mast cell growth factor (MGF), Kit ligand (Kitl), c-Kit ligand, steel factor and Kitlg. SCF is a pleiotropic cytokine (also as a dimeric molecule) that mediates regulatory effects on inflammation, tissue remodeling, and fibrosis through binding to and activating its receptor tyrosine kinase c-Kit (PMID: 1698553, PMID: 8630386, PMID: 23073628). SCF is the ligand of c-Kit, a member of the RTKs family. Molecular activity of RTKs is a primary stimulus of cell motility, and expression of the SCF/c-Kit axis is associated with cell migration (PMID: 28629522). SCF is a cytokine that synergizes the effects of Epo during erythropoiesis. Stem cell factor plays a well-described role in regulating the survival, proliferation, migration, and differentiation of hematopoietic progenitors, melanocytes, and germ cells (PMID: 36688460). More recent studies have demonstrated SCF expression in dermal and intestinal epithelial cells, and increased SCF expression has been reported in the inflamed mucosa of individuals with IBD and colonitis (PMID: 18200049, PMID: 30157426).

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