验证数据展示
经过测试的应用
| Positive IHC detected in | human liver tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23393-1-AP targets SCD in IHC, IF, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse, rat, chicken |
| 免疫原 |
CatNo: Ag13456 Product name: Recombinant human SCD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 155-355 aa of BC005807 Sequence: RDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGSTLDLSDLEAEKLVMFQRRYYKPGLLMMCFILPTLVPWYFWGETFQNSVFVATFLRYAVVLNATWLVNSAAHLFGYRPYDKNISPRENILVSLGAVGEGFHNYHHSFPYDYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKAAILARIKRTGDGN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | stearoyl-CoA desaturase (delta-9-desaturase) |
| 别名 | Acyl CoA desaturase, Delta(9) desaturase, FADS5, Fatty acid desaturase, MSTP008, SCD, SCD1, SCDOS, Stearoyl CoA desaturase |
| 计算分子量 | 355 aa, 41 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC005807 |
| 基因名称 | SCD |
| Gene ID (NCBI) | 6319 |
| RRID | AB_2744674 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00767 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SCD (stearoyl-CoA desaturase) is a 37-45 kDa (PMID:10946019) microsomal fatty acid monodesaturase, also commonly known as 9-desaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids. Five SCD genes (SCD1, SCD2, SCD3,SCD4 and SCD5) have been identified and characterized in mice and SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively, SCD3 is expressed exclusively in skin, whereas SCD4 is expressed predominantly in the heart. SCD1and SCD5 has been the only human SCD gene identified and characterized todate(PMID:15907797 ). The formation of homodimers and oligomers is an intrinsic property of SCD proteins, which may play an important role in regulating the half-life of the SCDenzymes, thus representing a novel regulatory mechanism for SCD enzymes, in addition to the transcriptional and post-translational regulations. A 32-kDa SCD1 degradation product identified may be caused by a major cleavage site at the C-terminus, thus representing a novel degradation product(PMID:15610069).
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Zeb1 mediates EMT/plasticity-associated ferroptosis sensitivity in cancer cells by regulating lipogenic enzyme expression and phospholipid composition | ||
Nat Commun The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition | ||
Nucleic Acids Res EWS-FLI1 regulates and cooperates with core regulatory circuitry in Ewing sarcoma. | ||
J Exp Clin Cancer Res Stearoyl-CoA desaturase-1 promotes colorectal cancer metastasis in response to glucose by suppressing PTEN.
| ||
J Clin Invest TRIM56 protects against nonalcoholic fatty liver disease by promoting the degradation of fatty acid synthase | ||
Cell Death Dis IGF2BP3 enhances lipid metabolism in cervical cancer by upregulating the expression of SCD |
