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SCCPDH Polyclonal antibody

SCCPDH Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 24941-1-AP

Print datasheet

Synonyms

CGI 49, NET11, RP11 439E19.2, SCCPDH

验证数据展示

  • WB analysis of COLO 320 using 24941-1-AP

    WB analysis of COLO 320 using 24941-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 24941-1-AP (SCCPDH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 24941-1-AP

    IP experiment of COLO 320 using 24941-1-AP

    IP result of anti-SCCPDH (IP:24941-1-AP, 3ug; Detection:24941-1-AP 1:300) with COLO 320 cells lysate 1700ug.

  • IF Staining of HepG2 using 24941-1-AP

    IF Staining of HepG2 using 24941-1-AP

    Immunofluorescent analysis of HepG2 cells using 24941-1-AP (SCCPDH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inCOLO 320 cells, HepG2 cells
Positive IP detected inCOLO 320 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24941-1-AP is SCCPDH Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IP, WB, ELISA
Tested Reactivity human, mouse
Immunogen SCCPDH fusion protein Ag11551 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name saccharopine dehydrogenase (putative)
Synonyms CGI 49, NET11, RP11 439E19.2, SCCPDH
Calculated Molecular Weight 429 aa, 47 kDa
Observed Molecular Weight 40-47 kDa
GenBank Accession NumberBC026185
Gene Symbol SCCPDH
Gene ID (NCBI) 51097
RRIDAB_2879811
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBX0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SCCPDH antibody 24941-1-APDownload protocol
IF protocol for SCCPDH antibody 24941-1-APDownload protocol
IP protocol for SCCPDH antibody 24941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols