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- KD/KO Validated
RPS4X Polyclonal antibody
RPS4X Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
IF/ICC, IHC, IP, WB,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, human liver tissue, MCF-7 cells, mouse pancreas tissue |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14799-1-AP targets RPS4X in WB, IP, IF, PLA, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, IP, WB,ELISA |
Cited Applications | WB, IF, PLA, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, Xenopus, frog |
Immunogen | RPS4X fusion protein Ag6513 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ribosomal protein S4, X-linked |
Synonyms | CCG2, DXS306, ribosomal protein S4, X linked, RPS4, RPS4X, SCAR, SCR10 |
Calculated Molecular Weight | 263 aa, 30 kDa |
Observed Molecular Weight | 30 kDa |
GenBank Accession Number | BC000472 |
Gene Symbol | RPS4X |
Gene ID (NCBI) | 6191 |
RRID | AB_2238567 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P62701 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for RPS4X antibody 14799-1-AP | Download protocol |
IHC protocol for RPS4X antibody 14799-1-AP | Download protocol |
IF protocol for RPS4X antibody 14799-1-AP | Download protocol |
IP protocol for RPS4X antibody 14799-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms. | ||
Nat Commun Excess ribosomal protein production unbalances translation in a model of Fragile X Syndrome. | ||
Elife Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons. | ||
Aging (Albany NY) Construction and external validation of a 5-gene random forest model to diagnose non-obstructive azoospermia based on the single-cell RNA sequencing of testicular tissue | ||
BMC Cancer Low expression of the X-linked ribosomal protein S4 in human serous epithelial ovarian cancer is associated with a poor prognosis.
| ||
Biomark Med Low level of the X-linked ribosomal protein S4 in human urothelial carcinomas is associated with a poor prognosis. |