验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, human liver tissue, mouse lung tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
15119-1-AP targets SCAMP2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag7190 Product name: Recombinant human SCAMP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-152 aa of BC001376 Sequence: MSAFDTNPFADPVDVNPFQDPSVTQLTNAPQGGLAEFNPFSETNAATTVPVTQLPGSSQPAVLQPSVEPTQPTPQAVVSAAQAGLLRQQEELDRKAAELERKERELQNTVANLHVRQNNWPPLPSWCPVKPCFYQDFSTEIPADYQRICKML 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | secretory carrier membrane protein 2 |
| 别名 | Secretory carrier-associated membrane protein 2 |
| 计算分子量 | 37 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC001376 |
| 基因名称 | SCAMP2 |
| Gene ID (NCBI) | 10066 |
| RRID | AB_2184772 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15127 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SCAMP2 functions in post-Golgi recycling pathways. It acts as a recycling carrier to the cell surface. It couples Arf6-stimulated PLD activity to exocytosis and links this process to formation of fusion pores. SCAMP2 is marker for secretory vesicles and tobacco BY-2 cells as a model system. The MW of SCAMP2 is 36-39 kDa with modification.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for SCAMP2 antibody 15119-1-AP | Download protocol |
| IF protocol for SCAMP2 antibody 15119-1-AP | Download protocol |
| IHC protocol for SCAMP2 antibody 15119-1-AP | Download protocol |
| IP protocol for SCAMP2 antibody 15119-1-AP | Download protocol |
| WB protocol for SCAMP2 antibody 15119-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma. | ||
Front Immunol Identification of SCAMP2 as a regulator of NOTCH signaling in cisplatin resistance through a novel prognostic model for bladder cancer |
