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SCAMP1 Polyclonal antibody

SCAMP1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 15327-1-AP

Print datasheet

Synonyms

SCAMP, SCAMP1, SCAMP37

验证数据展示

  • WB analysis of mouse brain using 15327-1-AP

    WB analysis of mouse brain using 15327-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15327-1-AP (SCAMP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 15327-1-AP

    IHC staining of mouse brain using 15327-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15327-1-AP (SCAMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 15327-1-AP

    IHC staining of mouse brain using 15327-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15327-1-AP (SCAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 15327-1-AP

    IHC staining of human pancreas cancer using 15327-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15327-1-AP (SCAMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 15327-1-AP

    IHC staining of human pancreas cancer using 15327-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15327-1-AP (SCAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 15327-1-AP

    IP experiment of mouse brain using 15327-1-AP

    IP result of anti-SCAMP1 (IP:15327-1-AP, 4ug; Detection:15327-1-AP 1:1000) with mouse brain tissue lysate 3200 ug.

经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, mouse pancreas tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15327-1-AP targets SCAMP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IP, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SCAMP1 fusion protein Ag3258 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name secretory carrier membrane protein 1
Synonyms SCAMP, SCAMP1, SCAMP37
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC034048
Gene Symbol SCAMP1
Gene ID (NCBI) 9522
RRIDAB_2184766
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15126
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SCAMP1 antibody 15327-1-APDownload protocol
IHC protocol for SCAMP1 antibody 15327-1-APDownload protocol
IP protocol for SCAMP1 antibody 15327-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Int J Mol Sci

Multiomics Analysis of Endocytosis upon HBV Infection and Identification of SCAMP1 as a Novel Host Restriction Factor against HBV Replication.

Authors - Tanzeel Yousaf
View Article
humanIHC

Cell Death Dis

MTSS1 and SCAMP1 cooperate to prevent invasion in breast cancer.

Authors - Jayakumar Vadakekolathu
View Article
humanWB

Aging (Albany NY)

Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma.

Authors - Feiyu Mao
View Article