验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HeLa cells, mouse pancreas tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15327-1-AP targets SCAMP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | SCAMP1 fusion protein Ag3258 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | secretory carrier membrane protein 1 |
| 别名 | SCAMP, SCAMP1, SCAMP37 |
| 计算分子量 | 38 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC034048 |
| 基因名称 | SCAMP1 |
| Gene ID (NCBI) | 9522 |
| RRID | AB_2184766 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15126 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SCAMP1 antibody 15327-1-AP | Download protocol |
| IHC protocol for SCAMP1 antibody 15327-1-AP | Download protocol |
| IP protocol for SCAMP1 antibody 15327-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Multiomics Analysis of Endocytosis upon HBV Infection and Identification of SCAMP1 as a Novel Host Restriction Factor against HBV Replication. | ||
Aging (Albany NY) Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma. | ||
J Cancer SCAMP1 silencing inhibits proliferation by attenuating multiple pro-survival signaling pathways in gastric cancer |
