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  • KD/KO Validated

SC65 Polyclonal antibody

SC65 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 15288-1-AP

Print datasheet

Synonyms

NOL55, Nucleolar autoantigen No55, P3H4, SC65



经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inmouse bladder tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15288-1-AP targets SC65 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen SC65 fusion protein Ag7480 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptonemal complex protein SC65
Synonyms NOL55, Nucleolar autoantigen No55, P3H4, SC65
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC001047
Gene Symbol SC65
Gene ID (NCBI) 10609
RRIDAB_2184611
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92791
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SC65, also named as Leprecan-like protein 4 or Nucleolar autoantigen No55, is a 437 amino acid protein, which belongs to the leprecan family. SC65 ia a protein of the interphase nucleolus and tumour-associated autoantigen in patients with prostate cancer.

实验方案

Product Specific Protocols
WB protocol for SC65 antibody 15288-1-APDownload protocol
IP protocol for SC65 antibody 15288-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

PLoS Genet

Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.

Authors - Melissa E Heard
  • KO Validated
human, mouseWB,IF

J Bone Miner Res

Sc65 is a novel endoplasmic reticulum protein that regulates bone mass homeostasis.

Authors - Gruenwald Katrin K
  • KD Validated
humanWB

Cancers (Basel)

P3H4 Promotes Malignant Progression of Lung Adenocarcinoma via Interaction with EGFR.

Authors - Chen Fang
  • KD Validated
mouseWB

PLoS Genet

Cyclophilin B control of lysine post-translational modifications of skin type I collagen.

Authors - Masahiko Terajima
humanWB,IHC

Aging (Albany NY)

Knockdown of P3H4 inhibits proliferation and invasion of bladder cancer.

Authors - Lin Hao
  • KD Validated
humanWB

Sci Rep

Transcriptome analysis of a dog model of congestive heart failure shows that collagen-related 2-oxoglutarate-dependent dioxygenases contribute to heart failure

Authors - Takahiro Isono