验证数据展示
经过测试的应用
| Positive WB detected in | mouse adipose tissue, mouse liver tissue, mouse small intestine tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84742-1-RR targets SC4MOL in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | SC4MOL fusion protein Ag34758 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | sterol-C4-methyl oxidase-like |
| 别名 | ERG25, DESP4, 241988B11 |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC107879 |
| 基因名称 | SC4MOL |
| Gene ID (NCBI) | 6307 |
| RRID | AB_3672127 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q15800 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SC4MOL(sterol C4-methyl oxidase-like), an intermediate enzyme in the cholesterol biosynthetic pathway, was included in the library based on its representation in a high confidence GEO transcriptional profiling dataset as a transcript that rapidly undergoes significant expression change in response to stimulation or inhibition of EGFR. Among the validated hits that increased cell killing by common EGFR antagonists, SC4MOL was consistently one of the most effective modulators. (PMID: 23125191)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SC4MOL antibody 84742-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |