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SBNO1 Polyclonal antibody

SBNO1 Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 25359-1-AP

Print datasheet

Synonyms

Monocyte protein 3, MOP 3, MOP3, SBNO1, Sno

验证数据展示

  • WB analysis using 25359-1-AP

    WB analysis using 25359-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25359-1-AP (SBNO1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 25359-1-AP

    IP experiment of Jurkat using 25359-1-AP

    IP result of anti-SBNO1 (IP:25359-1-AP, 4ug; Detection:25359-1-AP 1:3000) with Jurkat cells lysate 1400 ug.

  • IF Staining of HeLa using 25359-1-AP

    IF Staining of HeLa using 25359-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 25359-1-AP (SBNO1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, Raji cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25359-1-AP targets SBNO1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen SBNO1 fusion protein Ag19005 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name strawberry notch homolog 1 (Drosophila)
Synonyms Monocyte protein 3, MOP 3, MOP3, SBNO1, Sno
Calculated Molecular Weight 1393 aa, 154 kDa
Observed Molecular Weight 145-154 kDa
GenBank Accession NumberBC133704
Gene Symbol SBNO1
Gene ID (NCBI) 55206
RRIDAB_2880042
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA3KN83
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SBNO1, also named as Monocyte protein 3 or MOP3, is a 1393 amino acid protein, which belongs to the SBNO family. SBNO1 play an important role in development of the vertebrate central nervous system. SBNO1 has four isoforms with MW 154 kDa and 80 kDa.

实验方案

Product Specific Protocols
WB protocol for SBNO1 antibody 25359-1-APDownload protocol
IF protocol for SBNO1 antibody 25359-1-APDownload protocol
IP protocol for SBNO1 antibody 25359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Asian J Androl

Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice.

Authors - Cong Shen
View Article