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验证数据展示

  • WB analysis using 32117-1-AP

    WB analysis using 32117-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32117-1-AP (SBK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 32117-1-AP

    IF Staining of A549 using 32117-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SBK2 antibody (32117-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SBK2 Polyclonal antibody

SBK2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32117-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Sugen kinase 69 (SgK069), SH3 domain-binding kinase family member 2, SGK069, Serine/threonine-protein kinase SBK2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, Kasumi-1 cells
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32117-1-AP targets SBK2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SBK2 fusion protein Ag37233 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH3-binding domain kinase family, member 2
别名 Sugen kinase 69 (SgK069), SH3 domain-binding kinase family member 2, SGK069, Serine/threonine-protein kinase SBK2
计算分子量38 kDa
观测分子量30 kDa
GenBank蛋白编号NM_001101401
基因名称 SBK2
Gene ID (NCBI) 646643
RRIDAB_3670197
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP0C263
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH3 domain binding kinase family member 2 (SBK2, also known as SGK069), is an evolutionarily conserved putative serine/threonine protein kinase that plays a crucial role in cardiomyocyte differentiation, particularly in the formation and maintenance of sarcomeres. It is highly atrium-enriched and is muscle-specific, indicating its specialized function in cardiac muscle cells (PMID: 35587025). SBK2 may be useful for identifying and co-registering tumor borders during surgical resection (PMID: 23730413).

实验方案

Product Specific Protocols
WB protocol for SBK2 antibody 32117-1-APDownload protocol
IF protocol for SBK2 antibody 32117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols