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验证数据展示

  • WB analysis using 32277-1-AP

    WB analysis using 32277-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32277-1-AP (SAV1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 32277-1-AP

    IF Staining of A549 using 32277-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SAV1 antibody (32277-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of rat kidney using 32277-1-AP

    IHC staining of rat kidney using 32277-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 32277-1-AP (SAV1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 32277-1-AP

    IHC staining of mouse kidney using 32277-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32277-1-AP (SAV1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SAV1 Polyclonal antibody

SAV1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 32277-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

45 kDa WW domain protein, hWW45, Protein salvador homolog 1, SAV

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHT-29 cells, HUVEC cells, U2OS cells
Positive IHC detected inmouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32277-1-AP targets SAV1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag36278

Product name: Recombinant human SAV1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 208-383 aa of BC020537

Sequence: DWTMRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKKAQYRHPCAPSVPRYDQPPPVTYQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQIVKMYEAYRQALLTELENRKQRQQWYAQQHGKNF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 salvador homolog 1 (Drosophila)
别名 45 kDa WW domain protein, hWW45, Protein salvador homolog 1, SAV
计算分子量 383 aa, 45 kDa
观测分子量45 kDa
GenBank蛋白编号BC020537
基因名称 SAV1
Gene ID (NCBI) 60485
RRIDAB_3742564
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9H4B6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Salvador family WW domain‐containing 1 (SAV1, also known as WW45) is a human homologue of Salvador that contains two protein‐protein interaction modules known as WW domains and acts as a scaffolding protein (PMID: 28618450). SAV1 belongs to the core kinase components of Hippo signalling pathway for efficient MST1/2 activation. SAV1 represses the growth of colorectal cancer cells by inactivating Akt‐mTOR signalling in a YAP‐dependent manner (PMID: 29063833, 22185343).

实验方案

Product Specific Protocols
IF protocol for SAV1 antibody 32277-1-APDownload protocol
IHC protocol for SAV1 antibody 32277-1-APDownload protocol
WB protocol for SAV1 antibody 32277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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