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验证数据展示

  • WB analysis using 32277-1-AP

    WB analysis using 32277-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32277-1-AP (SAV1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 32277-1-AP

    IF Staining of A549 using 32277-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SAV1 antibody (32277-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of rat kidney using 32277-1-AP

    IHC staining of rat kidney using 32277-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 32277-1-AP (SAV1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 32277-1-AP

    IHC staining of mouse kidney using 32277-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32277-1-AP (SAV1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SAV1 Polyclonal antibody

SAV1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 32277-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

45 kDa WW domain protein, hWW45, Protein salvador homolog 1, SAV

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHT-29 cells, HUVEC cells, U2OS cells
Positive IHC detected inmouse kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32277-1-AP targets SAV1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag36278

Product name: Recombinant human SAV1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 208-383 aa of BC020537

Sequence: DWTMRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKKAQYRHPCAPSVPRYDQPPPVTYQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQIVKMYEAYRQALLTELENRKQRQQWYAQQHGKNF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 salvador homolog 1 (Drosophila)
别名 45 kDa WW domain protein, hWW45, Protein salvador homolog 1, SAV
计算分子量 383 aa, 45 kDa
观测分子量45 kDa
GenBank蛋白编号BC020537
基因名称 SAV1
Gene ID (NCBI) 60485
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9H4B6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Salvador family WW domain‐containing 1 (SAV1, also known as WW45) is a human homologue of Salvador that contains two protein‐protein interaction modules known as WW domains and acts as a scaffolding protein (PMID: 28618450). SAV1 belongs to the core kinase components of Hippo signalling pathway for efficient MST1/2 activation. SAV1 represses the growth of colorectal cancer cells by inactivating Akt‐mTOR signalling in a YAP‐dependent manner (PMID: 29063833, 22185343).

实验方案

Product Specific Protocols
IF protocol for SAV1 antibody 32277-1-APDownload protocol
IHC protocol for SAV1 antibody 32277-1-APDownload protocol
WB protocol for SAV1 antibody 32277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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