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验证数据展示

  • WB analysis using 30717-1-AP

    WB analysis using 30717-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30717-1-AP (SATB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 30717-1-AP

    IHC staining of human colon cancer using 30717-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30717-1-AP (SATB2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SATB2 Polyclonal antibody

SATB2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30717-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

DNA binding protein SATB2, DNA-binding protein SATB2, FLJ21474, FLJ32076, KIAA1034

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30717-1-AP targets SATB2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SATB2 fusion protein Ag33523 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SATB homeobox 2
别名 DNA binding protein SATB2, DNA-binding protein SATB2, FLJ21474, FLJ32076, KIAA1034
计算分子量 733 aa, 83 kDa
观测分子量 90 kDa
GenBank蛋白编号BC098136
基因名称 SATB2
Gene ID (NCBI) 23314
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPW6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SATB2, also named as KIAA1034, belongs to the CUT homeobox family. SATB2 binds to DNA at nuclear matrix- or scaffold-associated regions. STAB2 recognizes the sugar-phosphate structure of double-stranded DNA. SATB2 is a transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. SATB2 acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. It is required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. It is a repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. SATB2 may play an important role in palate formation. SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.

实验方案

Product Specific Protocols
WB protocol for SATB2 antibody 30717-1-APDownload protocol
IHC protocol for SATB2 antibody 30717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols