验证数据展示

WB analysis of human brain using 15400-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15400-1-AP (SATB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 15400-1-AP
IP result of anti-SATB1 (IP:15400-1-AP, 4ug; Detection:15400-1-AP 1:500) with HEK-293 cells lysate 2000ug.
WB analysis using 15400-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15400-1-AP (SATB1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
IHC staining of human oesophagus cancer using 15400-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SATB1 antibody included
IHC staining of human oesophagus cancer using 15400-1-AP
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SATB1 antibody included
IHC staining of human urothelial carcinoma using 15400-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SATB1 antibody included
IHC staining of mouse spleen using 15400-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SATB1 antibody included
IHC staining of mouse thymus using 15400-1-AP
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SATB1 antibody included
IHC staining of rat brain using 15400-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SATB1 antibody included
IHC staining of rat spleen using 15400-1-AP
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SATB1 antibody included
IHC staining of rat thymus using 15400-1-AP
Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SATB1 antibody included
IHC staining of rat thymus using 15400-1-AP
Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with SATB1 antibody included

Featured Product
KD/KO验证

SATB1 Polyclonal antibody

SATB1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15400-1-AP

产品说明书

引用文献(9)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA and More (2)

别名

Special AT-rich sequence-binding protein 1, DNA-binding protein SATB1

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (9)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	human brain tissue, HEK-293 cells, mouse brain tissue, mouse spleen tissue, rat brain tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human oesophagus cancer tissue, human urothelial carcinoma tissue, mouse spleen tissue, mouse thymus tissue, rat brain tissue, rat spleen tissue, rat thymus tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Planning an IHC experiment? We recommend our IHCeasy SATB1 Ready-To-Use IHC Kit. SATB1 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 3 publications below
WB	See 7 publications below
IP	See 1 publications below
CoIP	See 1 publications below
ChIP	See 1 publications below

产品信息

15400-1-AP targets SATB1 in WB, IHC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IP, ELISA Application Description
文献引用应用	WB, IP, CoIP, ChIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag7615 Product name: Recombinant human SATB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 415-763 aa of BC001744 Sequence: PKTASQSLLVNLRAMQNFLQLPEAERDRIYQDERERSLNAASAMGPAPLISTPPSRPPQVKTATIATERNGKPENNTMNINASIYDEIQQEMKRAKVSQALFAKVAATKSQGWLCELLRWKEDPSPENRTLWENLSMIRRFLSLPQPERDAIYEQESNAVHHHGDRPPHIIHVPAEQIQQQQQQQQQQQQQQQAPPPPQPQQQPQTGPRLPPRQPTVASPAESDEENRQKTRPRTKISVEALGILQSFIQDVGLYPDEEAIQTLSAQLDLPKYTIIKFFQNQRYYLKHHGKLKDNSGLEVDVAEYKEEELLKDLEESVQDKNTNTLFSVKLEEELSVEGNTDINTDLKD 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	SATB homeobox 1
别名	Special AT-rich sequence-binding protein 1, DNA-binding protein SATB1
计算分子量	86 kDa
观测分子量	86-100 kDa
GenBank蛋白编号	BC001744
基因名称	SATB1
Gene ID (NCBI)	6304
RRID	AB_2184321
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q01826
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Epigenetic modifications and dynamic changes in chromatin organization by organizer proteins have recently been shown to play an instrumental role in regulating cancer-promoting genes. Special AT-rich binding protein (SATB1) is a unique type of global regulator that integrates higher-order chromatin organization -withregulation of gene expression. [PMID:23076250,22998183,23121661] SATB1 is a T cell-enriched transcription factor and a chromatin organizer essential for controlling genes that participate in T-cell development and activation. It regulates gene expression by periodically anchoring matrix attachment regions to the nuclear matrix and directly recruiting chromatin-modifying factors. Depending on its posttranslational modifications, SATB1 activates or represses multiple genes.Its expression is regulated by interleukin-4 (IL4) during T helper-2(Th2) cell differentiation[PMID: 20522714]. The calculated molecular weight of SATB1 is 86 kDa, but modified SATB1 is about 100 kDa (PMID: 22879953).

实验方案

Product Specific Protocols
WB protocol for SATB1 antibody 15400-1-AP	Download protocol
IHC protocol for SATB1 antibody 15400-1-AP	Download protocol
IP protocol for SATB1 antibody 15400-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
		Dev Cell Folding Keratin Gene Clusters during Skin Regional Specification. Authors - Ya-Chen Liang View Article
human	WB,IP	Cell Rep SATB1 regulates 3D genome architecture in T cells by constraining chromatin interactions surrounding CTCF-binding sites Authors - Bao Wang KO Validated View Article
mouse,rat	WB	Pharmacol Res Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways. Authors - Jiale Cai View Article
mouse	WB	Mol Neurobiol SATB1/SLC7A11/HO-1 Axis Ameliorates Ferroptosis in Neuron Cells After Ischemic Stroke by Danhong Injection Authors - Sikai Zhan View Article
human	WB	Eur Rev Med Pharmacol Sci Effect of STAT3 mediated epigenetic regulation in pre-eclampsia: an analysis of trial data. Authors - X-L Zhang View Article
human	WB	Int J Med Sci SATB1 Knockdown Inhibits Proliferation and Invasion and Decreases Chemoradiation Resistance in Nasopharyngeal Carcinoma Cells by Reversing EMT and Suppressing MMP-9. Authors - Dongni Zhou KD Validated View Article

验证数据展示

WB analysis of human brain using 15400-1-AP

IP experiment of HEK-293 using 15400-1-AP

WB analysis using 15400-1-AP

IHC staining of human oesophagus cancer using 15400-1-AP

IHC staining of human oesophagus cancer using 15400-1-AP

IHC staining of human urothelial carcinoma using 15400-1-AP

IHC staining of mouse spleen using 15400-1-AP

IHC staining of mouse thymus using 15400-1-AP

IHC staining of rat brain using 15400-1-AP

IHC staining of rat spleen using 15400-1-AP

IHC staining of rat thymus using 15400-1-AP

IHC staining of rat thymus using 15400-1-AP

SATB1 Polyclonal antibody

SATB1 Polyclonal Antibody for WB, IHC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Folding Keratin Gene Clusters during Skin Regional Specification.

SATB1 regulates 3D genome architecture in T cells by constraining chromatin interactions surrounding CTCF-binding sites

Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.

SATB1/SLC7A11/HO-1 Axis Ameliorates Ferroptosis in Neuron Cells After Ischemic Stroke by Danhong Injection

Effect of STAT3 mediated epigenetic regulation in pre-eclampsia: an analysis of trial data.

SATB1 Knockdown Inhibits Proliferation and Invasion and Decreases Chemoradiation Resistance in Nasopharyngeal Carcinoma Cells by Reversing EMT and Suppressing MMP-9.