验证数据展示
经过测试的应用
| Positive WB detected in | rat liver tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
16246-1-AP targets SAT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag9179 Product name: Recombinant human SAT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-170 aa of BC011751 Sequence: MASVRIREAKEGDCGDILRLIRELAEFEKLSDQVKISEEALRADGFGDNPFYHCLVAEILPAPGKLLGPCVVGYGIYYFIYSTWKGRTIYLEDIYVMPEYRGQGIGSKIIKKVAEVALDKGCSQFRLAVLDWNQRAMDLYKALGAQDLTEAEGWHFFCFQGEATRKLAGK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | spermidine/spermine N1-acetyltransferase family member 2 |
| 别名 | Diamine acetyltransferase 2, SAT2, SSAT2 |
| 计算分子量 | 170 aa, 19 kDa |
| 观测分子量 | 19 kDa |
| GenBank蛋白编号 | BC011751 |
| 基因名称 | SAT2 |
| Gene ID (NCBI) | 112483 |
| RRID | AB_2254874 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96F10 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Spermidine/spermine N1-acetyltransferase 2 (SAT2) is a short-lived polyamine catabolic enzyme inducible by polyamines and polyamine analogues. Human SAT2 contains the highly conserved acetyl-CoA-binding domain and displays coactivator function to enhance NF-κB-dependent transcription and enhances TNFα-induced NF-κB activity (PMID: 17875644). Human SAT1 and SAT2 proteins, which share 46% amino acid sequence identity and are clearly homologous, both interact with HIF-1α and both promote the ubiquitination and degradation of HIF-1α (PMID: 17011643).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SAT2 antibody 16246-1-AP | Download protocol |
| IHC protocol for SAT2 antibody 16246-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Toxicol In Vitro Increased Sat2 expression is associated with busulfan-induced testicular Sertoli cell injury. | ||
Neuropharmacology A role of GABAA receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine |