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SAT1 Polyclonal antibody

SAT1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10708-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

SSAT 1, SSAT, Spermidine/spermine N(1)-acetyltransferase 1, SAT, Polyamine N-acetyltransferase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10708-1-AP targets SAT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 SAT1 fusion protein Ag1154 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spermidine/spermine N1-acetyltransferase 1
别名 SSAT 1, SSAT, Spermidine/spermine N(1)-acetyltransferase 1, SAT, Polyamine N-acetyltransferase 1
计算分子量 20 kDa
观测分子量 15-25 kDa
GenBank蛋白编号BC008424
基因名称 SAT1
Gene ID (NCBI) 6303
RRIDAB_2877739
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP21673
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Spermidine/spermine N1-acetyltransferase 1 (SAT1 or SSAT1) is the key regulatory enzyme in the catabolism of polyamines, catalyzing acetylation of spermidine or spermine to generate N1-acetyl spermidine or N1-acetyl spermine, and N1, N12-diacetylspermine. The cellular level of SAT1 is normally extremely low, but it is induced rapidly by a variety of stimuli, including polyamines, polyamine analogs, toxic chemicals, certain drugs, and growth factors. Downregulation of SAT1 has been reported in Epstein-Barr virus positive Burkitt's lymphoma cells.

实验方案

Product Specific Protocols
WB protocol for SAT1 antibody 10708-1-APDownload protocol
IHC protocol for SAT1 antibody 10708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IHC

Cell Metab

Urea cycle activation triggered by host-microbiota maladaptation driving colorectal tumorigenesis

Authors - Haoyan Chen
humanIHC

Proc Natl Acad Sci U S A

Polyamine pathway contributes to the pathogenesis of Parkinson disease.

Authors - Lewandowski Nicole M NM
ratWB,IHC

Mol Neurobiol

Electroacupuncture Alleviates Neuropathic Pain by Suppressing Ferroptosis in Dorsal Root Ganglion via SAT1/ALOX15 Signaling

Authors - Kexing Wan
mouseWB

Food Chem Toxicol

DEHP induces ferroptosis in testes via p38α-lipid ROS circulation and destroys the BTB integrity.

Authors - Ling Yang
mouseWB

Biochim Biophys Acta Mol Cell Res

Urea transporter B downregulates polyamines levels in melanoma B16 cells via p53 activation.

Authors - Jiajing Li
mouseWB

PLoS One

Relationships between hematopoiesis and hepatogenesis in the midtrimester fetal liver characterized by dynamic transcriptomic and proteomic profiles.

Authors - Yuanbiao Guo
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