验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10708-1-AP targets SAT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | SAT1 fusion protein Ag1154 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | spermidine/spermine N1-acetyltransferase 1 |
别名 | SSAT 1, SSAT, Spermidine/spermine N(1)-acetyltransferase 1, SAT, Polyamine N-acetyltransferase 1 |
计算分子量 | 20 kDa |
观测分子量 | 15-25 kDa |
GenBank蛋白编号 | BC008424 |
基因名称 | SAT1 |
Gene ID (NCBI) | 6303 |
RRID | AB_2877739 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P21673 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Spermidine/spermine N1-acetyltransferase 1 (SAT1 or SSAT1) is the key regulatory enzyme in the catabolism of polyamines, catalyzing acetylation of spermidine or spermine to generate N1-acetyl spermidine or N1-acetyl spermine, and N1, N12-diacetylspermine. The cellular level of SAT1 is normally extremely low, but it is induced rapidly by a variety of stimuli, including polyamines, polyamine analogs, toxic chemicals, certain drugs, and growth factors. Downregulation of SAT1 has been reported in Epstein-Barr virus positive Burkitt's lymphoma cells.
实验方案
Product Specific Protocols | |
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WB protocol for SAT1 antibody 10708-1-AP | Download protocol |
IHC protocol for SAT1 antibody 10708-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab Urea cycle activation triggered by host-microbiota maladaptation driving colorectal tumorigenesis | ||
Proc Natl Acad Sci U S A Polyamine pathway contributes to the pathogenesis of Parkinson disease. | ||
Mol Neurobiol Electroacupuncture Alleviates Neuropathic Pain by Suppressing Ferroptosis in Dorsal Root Ganglion via SAT1/ALOX15 Signaling | ||
Food Chem Toxicol DEHP induces ferroptosis in testes via p38α-lipid ROS circulation and destroys the BTB integrity. | ||
Biochim Biophys Acta Mol Cell Res Urea transporter B downregulates polyamines levels in melanoma B16 cells via p53 activation. | ||
PLoS One Relationships between hematopoiesis and hepatogenesis in the midtrimester fetal liver characterized by dynamic transcriptomic and proteomic profiles. |