验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31915-1-AP targets SASH3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag36154 Product name: Recombinant human SASH3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 230-380 aa of BC051881 Sequence: DVLPEEAVGHARPSRRQSKGKRPKPKTLHELLERIGLEEHTSTLLLNGYQTLEDFKELRETHLNELNIMDPQHRAKLLTAAELLLDYDTGSEEAEEGAESSQEPVAHTVSEPKVDIPRDSGCFEGSESGRDDAELAGTEEQLQGLSLAGAP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SAM and SH3 domain containing 3 |
| 别名 | CXorf9, SAM and SH3 domain-containing protein 3, SH3 protein expressed in lymphocytes homolog, SLY |
| 计算分子量 | 380 aa, 42 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC051881 |
| 基因名称 | SASH3 |
| Gene ID (NCBI) | 54440 |
| RRID | AB_3742493 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O75995 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sterile alpha motif (SAM) and Src homology-3 (SH3) domain-containing 3 (SASH3), also called SH3-containing lymphocyte protein (SLY1), is a putative adaptor protein that is postulated to play an important role in the organization of signaling complexes and propagation of signal transduction cascades in lymphocytes. The SASH3 gene is located on the X-chromosome, which plays an important role in human lymphocyte function and survival (PMID: 33876203).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SASH3 antibody 31915-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
