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  • Featured Product
  • KD/KO Validated

SART1 Polyclonal antibody

SART1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 22675-1-AP

Print datasheet

Synonyms

Ara1, Hom s 1, HOMS1, hSART 1, hSnu66, SART 1, SART1, SART1259, SNRNP110, Snu66, SNU66 homolog

验证数据展示

  • WB analysis of HeLa using 22675-1-AP

    WB analysis of HeLa using 22675-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22675-1-AP (SART1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 22675-1-AP

    IF Staining of HepG2 using 22675-1-AP

    Immunofluorescent analysis of HepG2 cells, using SART1 antibody 22675-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of mouse brain using 22675-1-AP

    IP experiment of mouse brain using 22675-1-AP

    IP result of anti-SART1 (IP:22675-1-AP, 4ug; Detection:22675-1-AP 1:1000) with mouse brain tissue lysate 5200ug.

  • IHC staining of mouse testis using 22675-1-AP

    IHC staining of mouse testis using 22675-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22675-1-AP (SART1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 22675-1-AP

    IHC staining of mouse testis using 22675-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22675-1-AP (SART1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22675-1-AP is SART1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen SART1 fusion protein Ag18478 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name squamous cell carcinoma antigen recognized by T cells
Synonyms Ara1, Hom s 1, HOMS1, hSART 1, hSnu66, SART 1, SART1, SART1259, SNRNP110, Snu66, SNU66 homolog
Calculated Molecular Weight 800 aa, 90 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC001058
Gene Symbol SART1
Gene ID (NCBI) 9092
RRIDAB_2879148
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO43290
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Squamous cell carcinoma antigen recognized by T cells (SART1), also known as U4/U6.U5 tri-snRNP-associated 110 kDa protein, is a ubiquitously expressed protein that is involved in mRNA splicing and the regulation of cell proliferation. It has been identified as regulator of c-FLIP and drug-induced activation of caspase 8. The SART1 gene encodes two proteins, the SART1(800) protein is located in the nucleus of the majority of proliferating cells, while the SART1(259) protein is located in the cytosol of epithelial cancers. Genetic variation in the SART1 gene may be associated with breast cancer development. This antibody recognizes the endogenous 800-amino acid SART1, which migrates with an apparent molecular mass of 110 kDa in SDS-PAGE. (PMID: 22027693; 9449708; 19377877; 11350945)

实验方案

Product Specific Protocols
WB protocol for SART1 antibody 22675-1-APDownload protocol
IHC protocol for SART1 antibody 22675-1-APDownload protocol
IF protocol for SART1 antibody 22675-1-APDownload protocol
IP protocol for SART1 antibody 22675-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF,WB

Theranostics

Suppressing Sart1 to modulate macrophage polarization by siRNA-loaded liposomes: a promising therapeutic strategy for pulmonary fibrosis.

Authors - Ting Pan
  • KD Validated
View Article