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验证数据展示

  • IF Staining of HepG2 using 17258-1-AP

    IF Staining of HepG2 using 17258-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 17258-1-AP (SARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 17258-1-AP

    WB analysis using 17258-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17258-1-AP (SARS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 17258-1-AP

    IHC staining of human lung cancer using 17258-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17258-1-AP (SARS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 17258-1-AP

    IHC staining of human lung cancer using 17258-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17258-1-AP (SARS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SARS2 Polyclonal antibody

SARS2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17258-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Serine--tRNA ligase, mitochondrial, Serine  tRNA ligase, SARSM, SARS, mtSerRS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse liver tissue, HepG2 cells, rat kidney tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17258-1-AP targets SARS2 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, RIP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag10913

Product name: Recombinant human SARS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 169-518 aa of BC042912

Sequence: KLPNQTHPDVPVGDESQARVLHMVGDKPVFSFQPRGHLEIGEKLDIIRQKRLSHVSGHRSYYLRGAGALLQHGLVNFTFNKLLRRGFTPMTVPDLLRGAVFEGCGMTPNANPSQIYNIDPARFKDLNLAGTAEVGLAGYFMDHTVAFRDLPVRMVCSSTCYRAETNTGQEPRGLYRVHHFTKVEMFGVTGPGLEQSSQLLEEFLSLQMEILTELGLHFRVLDMPTQELGLPAYRKFDIEAWMPGRGRFGEVTSASNCTDFQSRRLHIMFQTEAGELQFAHTVNATACAVPRLLIALLESNQQKDGSVLVPPALQSYLGTDRITAPTHVPLQYIGPNQPRKPGLPGQPAVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 seryl-tRNA synthetase 2, mitochondrial
别名 Serine--tRNA ligase, mitochondrial, Serine  tRNA ligase, SARSM, SARS, mtSerRS
计算分子量 518 aa, 58 kDa
观测分子量 53 kDa
GenBank蛋白编号BC042912
基因名称 SARS2
Gene ID (NCBI) 54938
RRIDAB_2238863
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NP81
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SARS2 antibody 17258-1-APDownload protocol
IHC protocol for SARS2 antibody 17258-1-APDownload protocol
IF protocol for SARS2 antibody 17258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNAHis mutation.

Authors - Shasha Gong
View Article
humanWB,RIP

Oncogene

NSUN2 regulates Wnt signaling pathway depending on the m5C RNA modification to promote the progression of hepatocellular carcinoma

Authors - Huiwu Xing
View Article