验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, HeLa cells, HT-1080 cells |
| Positive IHC detected in | human hepatocirrhosis tissue, human liver tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SAR1A Ready-To-Use IHC Kit. SAR1A primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15350-1-AP targets SAR1A in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag7596 Product name: Recombinant human SAR1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC003658 Sequence: MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYID 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SAR1 homolog A (S. cerevisiae) |
| 别名 | COPII associated small GTPase, GTP binding protein SAR1a, masra2, SAR1, SAR1 homolog A (S. cerevisiae), SAR1A, Sara, SARA1 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 23-25 kDa |
| GenBank蛋白编号 | BC003658 |
| 基因名称 | SAR1A |
| Gene ID (NCBI) | 56681 |
| RRID | AB_10667406 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NR31 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SAR1A antibody 15350-1-AP | Download protocol |
| WB protocol for SAR1A antibody 15350-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling. | ||
Redox Biol Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis | ||
Proc Natl Acad Sci U S A Atlastin-mediated membrane tethering is critical for cargo mobility and exit from the endoplasmic reticulum. | ||
J Biol Chem The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis. | ||
Clin Epigenetics Epigenetic silencing of TMEM176A activates ERK signaling in human hepatocellular carcinoma.
| ||
Biochem Biophys Res Commun Pull down assay for GTP-bound form of Sar1a reveals its activation during morphological differentiation. |
