验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293T cells, mouse kidney tissue |
| Positive IHC detected in | mouse kidney tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy PPP6R3/SAPS3 Ready-To-Use IHC Kit. PPP6R3/SAPS3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IP | See 1 publications below |
产品信息
16944-1-AP targets SAPS3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag10555 Product name: Recombinant human SAPS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-336 aa of BC020953 Sequence: VRERWETFCTSSLGETNKRNTVDLFGFNDEKFADQDDIGNVSFDRVSDINFTLNTNESGNIALFEACCKERIQQFDDGGSDEEDIWEEKHIAFTPESQRRSSSGSTDSEESTDSEEEDGAKQDLFEPSSANTEDKMEVDLSEPPNWSANFDVPMETTHGAPLDSVGSDVWSTEEPMPTKETGWASFSEFTSSLSTKDSLRSNSPVEMETSTEPMDPLTPSAAALAVQPEAAGSVAMEASSDGEEDAESTDKVTETVMNGGMKETLSLTVDAKTETAVFKSEEGKLSTSQDAACKDAEECPETAEAKCAAPRPPSSSPEQRTGQPSAPGDTSVNGPV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SAPS domain family, member 3 |
| 别名 | SAPLa, SAPL, PPP6R3, PP6R3, KIAA1558 |
| 计算分子量 | 873 aa, 98 kDa |
| 观测分子量 | 130-140 kDa |
| GenBank蛋白编号 | BC020953 |
| 基因名称 | SAPS3 |
| Gene ID (NCBI) | 55291 |
| RRID | AB_2183826 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5H9R7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SAPS3 antibody 16944-1-AP | Download protocol |
| IHC protocol for SAPS3 antibody 16944-1-AP | Download protocol |
| WB protocol for SAPS3 antibody 16944-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem A conserved acetylation switch enables pharmacological control of tubby-like protein stability. | ||
Cell Death Dis PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIPL to promote TNFα-mediated cell death | ||
