验证数据展示
经过测试的应用
| Positive WB detected in | human peripheral blood platelets, mouse lung tissue, rat lung tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16056-1-AP targets SAP30L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9024 Product name: Recombinant human SAP30L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-183 aa of BC009829 Sequence: MNGFSTEEDSREGPPAAPAAAAPGYGQSCCLIEDGERCVRPAGNASFSKRVQKSISQKKLKLDIDKSVRHLYICDFHKNFIQSVRNKRKRKTSDDGGDSPEHDTDIPEVDLFQLQVNTLRRYKRHYKLQTRPGFNKAQLAETVSRHFRNIPVNEKETLAYFIYMVKSNKSRLDQKSEGGKQLE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SAP30-like |
| 别名 | NS4ATP2, SAP30 like, SAP30L |
| 计算分子量 | 183 aa, 21 kDa |
| 观测分子量 | 21-25 kDa |
| GenBank蛋白编号 | BC009829 |
| 基因名称 | SAP30L |
| Gene ID (NCBI) | 79685 |
| RRID | AB_3085479 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HAJ7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SAP30L, also named as NS4ATP2, is one of the key proteins in a multi‐subunit protein complex involved in transcriptional regulation via histone deacetylation. SAP30L is an essential component of the Sin3A corepressor complex. The MW migrates to 26-30 kDa with phospho and SUMO modification and may generate complexes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SAP30L antibody 16056-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
