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验证数据展示

  • WB analysis using 29292-1-AP (same clone as 29292-1-PBS)

    WB analysis using 29292-1-AP (same clone as 29292-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29292-1-AP (DLG1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29292-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 29292-1-AP (same clone as 29292-1-PBS)

    IHC staining of human colon cancer using 29292-1-AP (same clone as 29292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29292-1-AP (DLG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29292-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DLG1 antibody included
  • IF Staining of HEK-293 using 29292-1-AP (same clone as 29292-1-PBS)

    IF Staining of HEK-293 using 29292-1-AP (same clone as 29292-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SAP 97 antibody (29292-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29292-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 29292-1-AP (same clone as 29292-1-PBS)

    WB analysis of HEK-293 using 29292-1-AP (same clone as 29292-1-PBS)

    WB result of SAP 97 antibody (29292-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SAP 97 transfected HEK-293 cells. This data was developed using the same antibody clone with 29292-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SAP 97 Polyclonal antibody, PBS Only

SAP 97 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29292-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DLG1, Disks large homolog 1, SAP-97, SAP97, hdlg

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DLG1 Ready-To-Use IHC Kit. DLG1 primary antibody included.

产品信息

29292-1-PBS targets SAP 97 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag31066

Product name: Recombinant human DLG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-229 aa of NM_001098424

Sequence: MPVRKQDTQRALHLLEEYRSKLSQTEDRQLRSSIERVINIFQSNLFQALIDIQEFYEVTLLDNPKCIDRSKPSEPIQPVNTWEISSLPSSTVTSETLPSSLSPSVEKYRYQDEDTPPQEHISPQITNEVIGPELVHVSEKNLSEIENVHGFVSHSHISPIKPTEAVLPSPPTVPVIPVLPVPAENTVILPTIPQANPPPVLVNTDSLETPTYVNGTDADYEYEEITLER

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 discs, large homolog 1 (Drosophila)
别名 DLG1, Disks large homolog 1, SAP-97, SAP97, hdlg
计算分子量 100 kDa
观测分子量100-130 kDa
GenBank蛋白编号NM_001098424
基因名称 SAP97
Gene ID (NCBI) 1739
RRIDAB_2918273
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12959
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DLG1, also naemd as SAP97, hDlg and DLGH1, belongs to the MAGUK family. It is essential multidomain scaffolding protein required for normal development. DLG1 may play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. It regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. DLG1 is a functional regulator of Kv1.5 channel. The DLG1-MPP7 complex promotes epithelial cell polarity and tight junction formation. The antibody is specific to DLG1.

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