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  • Featured Product
  • KD/KO Validated

SAMSN1 Polyclonal antibody

SAMSN1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13063-1-AP

Print datasheet

Synonyms

HACS1, NASH1, SAMSN1, SASH2, SH3 SAM adaptor protein, SH3D6B

验证数据展示

  • IHC staining of human gliomas using 13063-1-AP

    IHC staining of human gliomas using 13063-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13063-1-AP (SAMSN1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SAMSN1 antibody included
  • IHC staining of human gliomas using 13063-1-AP

    IHC staining of human gliomas using 13063-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13063-1-AP (SAMSN1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SAMSN1 antibody included
  • IP experiment of K-562 using 13063-1-AP

    IP experiment of K-562 using 13063-1-AP

    IP result of anti-SAMSN1 (IP:13063-1-AP, 4ug; Detection:13063-1-AP 1:1000) with K-562 cells lysate 4000 ug.

  • WB analysis of K-562 using 13063-1-AP

    WB analysis of K-562 using 13063-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13063-1-AP (SAMSN1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 13063-1-AP

    IF Staining of HepG2 using 13063-1-AP

    Immunofluorescent analysis of HepG2 cells using 13063-1-AP (SAMSN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SAMSN1 Ready-To-Use IHC Kit. SAMSN1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13063-1-AP targets SAMSN1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, rat
Cited Reactivityhuman, mouse
Immunogen SAMSN1 fusion protein Ag3755 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SAM domain, SH3 domain and nuclear localization signals 1
Synonyms HACS1, NASH1, SAMSN1, SASH2, SH3 SAM adaptor protein, SH3D6B
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42-50 kDa
GenBank Accession NumberBC029112
Gene Symbol SAMSN1
Gene ID (NCBI) 64092
RRIDAB_2877910
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NSI8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SAMSN1 antibody 13063-1-APDownload protocol
IHC protocol for SAMSN1 antibody 13063-1-APDownload protocol
IF protocol for SAMSN1 antibody 13063-1-APDownload protocol
IP protocol for SAMSN1 antibody 13063-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Stem Cells Int

Lama2 And Samsn1 Mediate the Effects of Brn4 on Hippocampal Neural Stem Cell Proliferation and Differentiation

Authors - Lei Zhang
  • KD Validated
View Article
humanIHC

Ann Surg Oncol

Suppression of SAMSN1 Expression is Associated with the Malignant Phenotype of Hepatocellular Carcinoma.

Authors - Satoshi Sueoka
View Article
humanIHC

Oncol Lett

Prognostic relevance of SAMSN1 expression in gastric cancer.

Authors - Mitsuro Kanda
View Article