验证数据展示
经过测试的应用
| Positive WB detected in | rat kidney tissue, COLO 320 cells |
| Positive IHC detected in | human breast cancer tissue, mouse spleen tissue, human brain tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | COLO 320 cells, 3T3-L1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25173-1-AP targets SAMD9L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | SAMD9L fusion protein Ag18541 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sterile alpha motif domain containing 9-like |
| 别名 | C7orf6, DRIF2, KIAA2005, SAMD9L, UEF, UEF1 |
| 计算分子量 | 1584 aa, 185 kDa |
| 观测分子量 | 160-180 kDa |
| GenBank蛋白编号 | BC127118 |
| 基因名称 | SAMD9L |
| Gene ID (NCBI) | 219285 |
| RRID | AB_2879940 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IVG5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sterile alpha motif domain-containing protein 9-like(SAMD9L), also named as C7orf6, DRIF2, KIAA2005 and UEF, is a cytoplasmic protein that acts as a tumor suppressor but also plays a key role in cell proliferation and the innate immune response to viral infection. SAMD9L protein is an endosome fusion facilitator which causes myeloid malignancies in mice similar to human diseases with monosomy 7. The SAMD9L protein also appears to play an important role in the brain, particularly the part of the brain that coordinates movement (the cerebellum). SAMD9L has two isoforms with MW 185 kDa and 47 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SAMD9L antibody 25173-1-AP | Download protocol |
| IHC protocol for SAMD9L antibody 25173-1-AP | Download protocol |
| IF protocol for SAMD9L antibody 25173-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Med Clinical evolution, genetic landscape and trajectories of clonal hematopoiesis in SAMD9/SAMD9L syndromes. | ||
PLoS Pathog A paralogous pair of mammalian host restriction factors form a critical host barrier against poxvirus infection.
| ||
Immunobiology Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations | ||
Brain Commun New spinocerebellar ataxia subtype caused by SAMD9L mutation triggering mitochondrial dysregulation (SCA49). | ||
J Inflamm Res Integrated Analysis Identifies Upregulated SAMD9L as a Potential Biomarker Correlating with the Severity of Primary Sjögren's Syndrome |
