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验证数据展示

  • WB analysis using 22786-1-AP

    WB analysis using 22786-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22786-1-AP (SAMD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 22786-1-AP

    WB analysis of HEK-293 using 22786-1-AP

    WB result of SAMD9 antibody (22786-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SAMD9 transfected HEK-293 cells.

  • IHC staining of human ovary cancer using 22786-1-AP

    IHC staining of human ovary cancer using 22786-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22786-1-AP (SAMD9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • KD/KO验证

SAMD9 Polyclonal antibody

SAMD9 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22786-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C7orf5, DRIF1, KIAA2004, NFTC, OEF1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22786-1-AP targets SAMD9 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18787

Product name: Recombinant human SAMD9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-178 aa of BC132775

Sequence: MAKQLNLPENTDDWTKEDVNQWLESHKIDQKHREILTEQDVNGAVLKWLKKEHLVDMGITHGPAIQIEELFKELRKTAIEDSIQTSKMGKPSKNAPKDQTVSQKERRETSKQKQKGKENPDMANPSAMSTTAKGSKSLKVELIEDKIDYTKERQPSIDLTCVSYPFDEFSNPYRYKLD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sterile alpha motif domain containing 9
别名 C7orf5, DRIF1, KIAA2004, NFTC, OEF1
计算分子量 1589 aa, 184 kDa
观测分子量 148 kDa
GenBank蛋白编号BC132775
基因名称 SAMD9
Gene ID (NCBI) 54809
RRIDAB_2879168
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5K651
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sterile alpha motif domain-containing protein 9(SAMD9), aslo named as C7orf5, DRIF1, KIAA2004, OEF1, plays a role in growth factor signal transduction. SAMD9 is a ubiquitously expressed cytoplasmic protein encoded by two juxtaposed single-exon genes on chromosome 7q21(PMID: 34675305). Overexpression of SAMD9 protein in tissue culture results in reduction of cell proliferation rate and invasion index, and increasing activity of caspase 3(PMID: 25450373). An important paralog of this gene is SAMD9L. Observed MW of SAMD9 is from 148 kDa(PMID: 21248034).

实验方案

Product Specific Protocols
IHC protocol for SAMD9 antibody 22786-1-APDownload protocol
WB protocol for SAMD9 antibody 22786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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