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验证数据展示

  • WB analysis of HeLa using 17723-1-AP

    WB analysis of HeLa using 17723-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17723-1-AP (SAMD4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 17723-1-AP

    IF Staining of HepG2 using 17723-1-AP

    Immunofluorescent analysis of HepG2 cells, using SAMD4B antibody 17723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human urothelial carcinoma using 17723-1-AP

    IHC staining of human urothelial carcinoma using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SAMD4B antibody included
  • IHC staining of human urothelial carcinoma using 17723-1-AP

    IHC staining of human urothelial carcinoma using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SAMD4B antibody included
  • IHC staining of human thyroid cancer using 17723-1-AP

    IHC staining of human thyroid cancer using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SAMD4B antibody included
  • IHC staining of human thyroid cancer using 17723-1-AP

    IHC staining of human thyroid cancer using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SAMD4B antibody included
  • IHC staining of mouse kidney using 17723-1-AP

    IHC staining of mouse kidney using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SAMD4B antibody included
  • IHC staining of mouse pancreas using 17723-1-AP

    IHC staining of mouse pancreas using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SAMD4B antibody included
  • IHC staining of rat kidney using 17723-1-AP

    IHC staining of rat kidney using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SAMD4B antibody included
  • IHC staining of rat pancreas using 17723-1-AP

    IHC staining of rat pancreas using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SAMD4B antibody included
  • IHC staining of rat small intestine using 17723-1-AP

    IHC staining of rat small intestine using 17723-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 17723-1-AP (SAMD4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SAMD4B antibody included
  • Featured Product
  • KD/KO验证

SAMD4B Polyclonal antibody

SAMD4B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17723-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

hSmaug2, Protein Smaug homolog 2, SAM domain-containing protein 4B, Smaug 2, SMAUG2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman urothelial carcinoma tissue, human thyroid cancer tissue, mouse kidney tissue, mouse pancreas tissue, rat kidney tissue, rat pancreas tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SAMD4B Ready-To-Use IHC Kit. SAMD4B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17723-1-AP targets SAMD4B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag12113

Product name: Recombinant human SAMD4B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 343-694 aa of BC054518

Sequence: KGARHKIALSIQKLRERQSVLKSLEKDVLEGGNLRNALQELQQIIITPIKAYSVLQATVAAATTTPTAKDGAPGEPPLPGAEPPLAHPGTDKGTEAKDPPAVENYPPPPAPAPTDGSEPAPAPVADGDIPSQFTRVMGKVCTQLLVSRPDEENITSYLQLIEKCLTHEAFTETQKKRLLSWKQQVLKLLRTFPRKAALEMQNYRQQKGWAFGSNSLPIAGSVGMGVARRTQRQFPMPPRALPPGRMGLLSPSGIGGVSPRHALTSPSLGGQGRQNLWFANPGGSNSMPSQSRSSVQRTHSLPVHSSPQAILMFPPDCPVPGPDLEINPTLESLCLSMTEHALGDGTDKTSTI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sterile alpha motif domain containing 4B
别名 hSmaug2, Protein Smaug homolog 2, SAM domain-containing protein 4B, Smaug 2, SMAUG2
计算分子量 694 aa, 75 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC054518
基因名称 SAMD4B
Gene ID (NCBI) 55095
RRIDAB_2183381
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5PRF9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sterile alpha motif domain-containing protein 4B (SAMD4B) is also named as Smaug2. SAMD4B is a mammalian homologue of the Smaug protein of Drosophila melanogaster and can participate in the process of regulating RNA stability as a posttranscriptional inhibitor (PMID: 23592795). SAMD4B is expressed in most human embryos and adult tissue (PMID: 23592795). SAMD4B, especially its expression level, could affect the immune microenvironment (PMID: 38886351).

实验方案

Product Specific Protocols
WB protocol for SAMD4B antibody 17723-1-APDownload protocol
IHC protocol for SAMD4B antibody 17723-1-APDownload protocol
IF protocol for SAMD4B antibody 17723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Mol Immunol

SAMD4 family members suppress human hepatitis B virus by directly binding to the Smaug recognition region of viral RNA.

Authors - Yuze Wang
  • KO Validated
View Article
humanWB,IF

Mol Med Rep

miR‑451 suppresses the malignant characteristics of colorectal cancer via targeting SAMD4B.

Authors - Chunrong Wu
View Article