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Sam50 Monoclonal antibody

Sam50 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rat, Mouse, rabbit

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H6H9

Cat No : 67425-1-Ig

Print datasheet

Synonyms

CGI 51, FLJ35825, FLJ42905, FLJ99036, OMP85, Sam50, SAMM50, TOB55, TRG 3, TRG3, YNL026W

验证数据展示

  • WB analysis using 67425-1-Ig

    WB analysis using 67425-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67425-1-Ig (Sam50 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67425-1-Ig

    IHC staining of human liver cancer using 67425-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67425-1-Ig (SAMM50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67425-1-Ig

    IHC staining of human liver cancer using 67425-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67425-1-Ig (SAMM50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 67425-1-Ig

    IF Staining of HeLa using 67425-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sam50 antibody (67425-1-Ig, Clone: 2H6H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inA375 cells, rabbit heart tissue, HSC-T6 cells, NIH/3T3 cells, pig heart tssue, rat heart tissue, mouse heart tissue
Positive IHC detected inhuman liver cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67425-1-Ig targets Sam50 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Pig, Rat, Mouse, rabbit samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, Pig, Rat, Mouse, rabbit
Immunogen Sam50 fusion protein Ag15155 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sorting and assembly machinery component 50 homolog (S. cerevisiae)
Synonyms CGI 51, FLJ35825, FLJ42905, FLJ99036, OMP85, Sam50, SAMM50, TOB55, TRG 3, TRG3, YNL026W
Calculated Molecular Weight 469 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC007830
Gene Symbol SAMM50
Gene ID (NCBI) 25813
RRIDAB_2882664
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y512
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sam50, encoded by the SAMM50 gene, is an essential protein of the mitochondrial outer membrane (OMM). It contains a beta-barrel domain conserved from bacteria to man (PMID: 14570913). Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of beta-barrel proteins in the OMM (PMID: 22252321). It is crucial for maintaining mitochondrial shape, morphology of mitochondrial cristae, and assembly of the mitochondrial respiratory chain complexes (PMID: 26587038; 22252321; 25781180). Polymorphisms in the SAMM50 gene have been associated with development and progression of nonalcoholic fatty liver disease (PMID: 23535911).

实验方案

Product Specific Protocols
WB protocol for Sam50 antibody 67425-1-IgDownload protocol
IHC protocol for Sam50 antibody 67425-1-IgDownload protocol
IF protocol for Sam50 antibody 67425-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols