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  • Featured Product
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SALL4 Polyclonal antibody

SALL4 Polyclonal Antibody for WB, IP, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IP, IF/ICC, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 24500-1-AP

Print datasheet

Synonyms

DRRS, HSAL4, sal like 4 (Drosophila), Sal like protein 4, SALL4, Zinc finger protein SALL4, ZNF797

验证数据展示

  • WB analysis of HepG2 using 24500-1-AP

    WB analysis of HepG2 using 24500-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24500-1-AP (SALL4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 24500-1-AP

    IHC staining of human testis using 24500-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 24500-1-AP (SALL4 Antibody) at dilution of 1:50.

  • IP experiment of HepG2 using 24500-1-AP

    IP experiment of HepG2 using 24500-1-AP

    IP result of anti-SALL4 (IP:24500-1-AP, 4ug; Detection:24500-1-AP 1:600) with HepG2 cells lysate 3600ug.

  • IF Staining of Caco-2 using 24500-1-AP

    IF Staining of Caco-2 using 24500-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SALL4 antibody (24500-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman testis tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCaco-2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24500-1-AP targets SALL4 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications WB, IP, IF/ICC, IHC, ELISA
Cited ApplicationsWB, IF, IHC, CoIP
Tested Reactivity human, rat, mouse
Cited Reactivityhuman
Immunogen SALL4 fusion protein Ag17480 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sal-like 4 (Drosophila)
Synonyms DRRS, HSAL4, sal like 4 (Drosophila), Sal like protein 4, SALL4, Zinc finger protein SALL4, ZNF797
Calculated Molecular Weight 1053 aa, 112 kDa
Observed Molecular Weight 66-75 kDa
GenBank Accession NumberBC111714
Gene Symbol SALL4
Gene ID (NCBI) 57167
RRIDAB_2879576
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJQ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sal-like protein 4 (SALL4), also named Zinc finger protein 797, contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is a transcription factor that exists in two splice isoforms, SALL4a and SALL4b, and regulates transcription in embryonic stem cells, hematopoiesis, and acute myeloid leukemia. SALL4 is activated in fetal liver tissue, silenced in adult liver tissue, and reexpressed as an oncofetal protein in hepatocellular carcinoma (PMID: 26892498, 23758232). SALL4 has two isoforms with predicted molecular weights of 112 kDa (SALL4A) and 66 kDa (unmodified SALL4B), and SALL4B was found to be the dominant endogenous isoform in HCC cell lines. Ectopically expressed SALL4B migrated at 95 kDa modified by ubiquitination, 130 kDa modified by sumoylation (PMID: 23012367).

实验方案

Product Specific Protocols
WB protocol for SALL4 antibody 24500-1-APDownload protocol
IHC protocol for SALL4 antibody 24500-1-APDownload protocol
IF protocol for SALL4 antibody 24500-1-APDownload protocol
IP protocol for SALL4 antibody 24500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Sci Rep

Hepatocellular Carcinoma-propagating Cells are Detectable by Side Population Analysis and Possess an Expression Profile Reflective of a Primitive Origin.

Authors - Honghai Xia
View Article
humanIHC,WB

Cell Signal

miR-296-5p suppresses stem cell potency of hepatocellular carcinoma cells via regulating Brg1/Sall4 axis.

Authors - Dong-Min Shi
View Article
humanWB

Onco Targets Ther

PMID: 31632072

Authors - Chiung-Chi Cheng
View Article
humanWB

Sci Rep

MicroRNA-33b Inhibits Breast Cancer Metastasis by Targeting HMGA2, SALL4 and Twist1.

Authors - Yancheng Lin
View Article
humanWB

Pathol Int

Salt-like transcription factor 4 promotes laryngeal cancer progression through transcriptional activation of ubiquitin-specific protease 21 to stabilize Yin Yang 1

Authors - Qing-Dong Wang
View Article
humanWB,IHC,IF

J Mol Histol

Expression and clinical significance of SALL4 and β-catenin in colorectal cancer.

Authors - Liliang Hao
View Article