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验证数据展示

  • IHC staining of human placenta using 27761-1-AP

    IHC staining of human placenta using 27761-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27761-1-AP (SALL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 27761-1-AP

    IHC staining of mouse testis using 27761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27761-1-AP (SALL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 27761-1-AP

    IHC staining of mouse testis using 27761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27761-1-AP (SALL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 27761-1-AP

    IF Staining of mouse brain using 27761-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SALL1 antibody (27761-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 27761-1-AP

    IF Staining of mouse brain using 27761-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SALL1 antibody (27761-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SALL1 Polyclonal antibody

SALL1 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 27761-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF-P, ELISA

别名

HSAL1, Sal 1, Sal like protein 1, SAL1, TBS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse testis tissue, human placenta tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27761-1-AP targets SALL1 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SALL1 fusion protein Ag26766 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sal-like 1 (Drosophila)
别名 HSAL1, Sal 1, Sal like protein 1, SAL1, TBS
计算分子量 140 kDa
观测分子量140 kDa
GenBank蛋白编号NM_001127892
基因名称 SALL1
Gene ID (NCBI) 6299
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NSC2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SALL1(Sal-like protein 1), also known as SAL1. It is located in the cytoplasm and nucleus. The protein is mainly expressed in the kidney. Transcriptional repressor involved in organogenesis. The protein plays an essential role in ureteric bud invasion during kidney development. The molecular weight of SALL1 is 140 kDa.

实验方案

Product Specific Protocols
IHC protocol for SALL1 antibody 27761-1-APDownload protocol
IF protocol for SALL1 antibody 27761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols