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验证数据展示

  • WB analysis of mouse eye using 23546-1-AP

    WB analysis of mouse eye using 23546-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 23546-1-AP (Retinal S antigen antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of rat eye using 23546-1-AP

    IF Staining of rat eye using 23546-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using Retinal S antigen antibody (23546-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Retinal S antigen Polyclonal antibody

Retinal S antigen Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 23546-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

SAG, 48 kDa protein, Retinal S-antigen, Rod photoreceptor arrestin, S AG

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse eye tissue
Positive IF-P detected inrat eye tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23546-1-AP targets Retinal S antigen in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag20318

Product name: Recombinant human SAG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 332-405 aa of BC156656

Sequence: QIKVKLTVSGFLGELTSSEVATEVPFRLMHPQPEDPAKESYQDANLVFEEFARHNLKDAGEAEEGKRDKNDVDE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 S-antigen; retina and pineal gland (arrestin)
别名 SAG, 48 kDa protein, Retinal S-antigen, Rod photoreceptor arrestin, S AG
计算分子量 405 aa, 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC156656
基因名称 Retinal S antigen
Gene ID (NCBI) 6295
RRIDAB_2879294
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10523
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Retinal S antigen antibody 23546-1-APDownload protocol
IF protocol for Retinal S antigen antibody 23546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols