验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
13874-1-AP targets S1PR5/EDG8 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4928 Product name: Recombinant human S1PR5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 214-398 aa of BC034703 Sequence: RIYCQVRANARRLPARPGTAGTTSTRARRKPRSLALLRTLSVVLLAFVACWGPLFLLLLLDVACPARTCPVLLQADPFLGLAMANSLLNPIIYTLTNRDLRHALLRLVCCGRHSCGRDPSGSQQSASAAEASGGLRRCLPPGLDGSFSGSERSSPQRDGLDTSGSTGSPGAPTAARTLVSEPAAD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sphingosine-1-phosphate receptor 5 |
| 别名 | Edg 8, EDG8, S1P receptor 5, S1P receptor Edg 8, S1P5, S1PR5, S1PR5/EDG8, SPPR 1, SPPR 2 |
| 计算分子量 | 398 aa, 42 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC034703 |
| 基因名称 | S1PR5 |
| Gene ID (NCBI) | 53637 |
| RRID | AB_2182638 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H228 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sphingosine 1-phosphate (S1P) is a biologically active lysophospholipid that elicits diverse physiological effects on most types of cells and tissues via five G protein-coupled receptors, S1PR1 to S1PR5. S1PRs differ in their tissue expression and cell lineage: S1PR1-3 are expressed throughout the immune, central nervous system (CNS), and cardiovascular system; S1PR4 is primarily expressed in lymphoid tissue; and S1PR5 is a predominant receptor in CNS white matter, spleen and natural killer cells (PMID: 21707490). S1PR5 (also known as EDG8) has been involved in the trafficking of NK cells and monocytes (PMID: 21911833; 23519784).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for S1PR5/EDG8 antibody 13874-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS One Sphingosine 1-phosphate (S1P) reduces hepatocyte growth factor-induced migration of hepatocellular carcinoma cells via S1P receptor 2. | ||
Drug Des Devel Ther Network Pharmacology and Experimental Verification to Explore the Potential Mechanism of Yin-Huo-Tang for Lung Adenocarcinoma Recurrence. | ||
Sci Rep Transcriptional signature of rapidly responding NK cells reveals S1P5 and CXCR4 as anti-tumor response disruptors |
