验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55204-1-AP targets S1PR3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sphingosine-1-phosphate receptor 3 |
| 别名 | LPB3, Endothelial differentiation G-protein coupled receptor 3, EDG3, EDG 3, C9orf47 |
| 计算分子量 | 42 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | NM_005226 |
| 基因名称 | S1PR3 |
| Gene ID (NCBI) | 1903 |
| RRID | AB_3670241 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99500 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
S1PR3 (sphingosine-1-phosphate receptor 3), also known as as S1P3 or EDG3, is one of five G-protein-coupled receptors for sphingosine-1-phosphate (S1P). S1P1 and its receptors have important regulatory functions in normal physiology and disease processes, particularly involving the immune, central nervous, and cardiovascular systems (PMID: 21339489). S1PR3 has a role in the development of a stable and mature vascular system during embryonic development (PMID: 15138255).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for S1PR3 antibody 55204-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |