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验证数据展示

  • IHC staining of mouse small intestine using 18340-1-AP

    IHC staining of mouse small intestine using 18340-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 18340-1-AP (S100G/Calbindin-D9k antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse small intestine using 18340-1-AP

    IHC staining of mouse small intestine using 18340-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 18340-1-AP (S100G/Calbindin-D9k antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

S100G/Calbindin-D9k Polyclonal antibody

S100G/Calbindin-D9k Polyclonal Antibody for IHC, ELISA
Cat No. 18340-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

CABP, CABP1, CABP9K, CALB3, Calbindin D9k, Protein S100 G, S100 calcium binding protein G, S100D, S100G, S100G/Calbindin-D9k

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18340-1-AP targets S100G/Calbindin-D9k in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13109

Product name: Recombinant human S100G protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-79 aa of BC112174

Sequence: MSTKKSPEELKRIFEKYAAKEGDPDQLSKDELKLLIQAEFPSLLKGPNTLDDLFQELDKNGDGEVSFEEFQVLVKKISQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 S100 calcium binding protein G
别名 CABP, CABP1, CABP9K, CALB3, Calbindin D9k, Protein S100 G, S100 calcium binding protein G, S100D, S100G, S100G/Calbindin-D9k
计算分子量 79 aa, 9 kDa
GenBank蛋白编号BC112174
基因名称 S100G
Gene ID (NCBI) 795
RRIDAB_3085561
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP29377
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S100G also named calbindin D9K, is a vitamin D-dependent calcium-binding protein. This cytosolic protein belongs to a family of calcium-binding proteins that includes calmodulin, parvalbumin, troponin C, and S100 protein. In the intestine, the protein is vitamin D-dependent and its expression correlates with calcium transport activity. The protein may increase Ca2+ absorption by buffering Ca2+ in the cytoplasm and increase ATP-dependent Ca2+ transport in duodenal basolateral membrane vesicles.

实验方案

Product Specific Protocols
IHC protocol for S100G/Calbindin-D9k antibody 18340-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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