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验证数据展示

  • IHC staining of human tonsillitis using 66853-1-Ig (same clone as 66853-1-PBS)

    IHC staining of human tonsillitis using 66853-1-Ig (same clone as 66853-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66853-1-Ig (S100A8 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66853-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A8 antibody included
  • IHC staining of human tonsillitis using 66853-1-Ig (same clone as 66853-1-PBS)

    IHC staining of human tonsillitis using 66853-1-Ig (same clone as 66853-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66853-1-Ig (S100A8 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66853-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A8 antibody included
  • IF Staining of human tonsillitis using 66853-1-Ig (same clone as 66853-1-PBS)

    IF Staining of human tonsillitis using 66853-1-Ig (same clone as 66853-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using S100A8 antibody (66853-1-Ig, Clone: 1G11C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66853-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66853-1-Ig (same clone as 66853-1-PBS)

    IF Staining of human tonsillitis using 66853-1-Ig (same clone as 66853-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using S100A8 antibody (66853-1-Ig, Clone: 1G11C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66853-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66853-1-Ig (same clone as 66853-1-PBS)

    IHC staining of human liver cancer using 66853-1-Ig (same clone as 66853-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66853-1-Ig (S100A8 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66853-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with S100A8 antibody included

S100A8 Monoclonal antibody, PBS Only

S100A8 Monoclonal Antibody for IHC, IF-P, Indirect ELISA
Cat No. 66853-1-PBS

产品说明书

CloneNo. 1G11C8

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IHC, IF-P, Indirect ELISA

别名

CFAG, Calprotectin L1L subunit, Calgranulin-A, Calgranulin A, CAGA

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy S100A8 Ready-To-Use IHC Kit. S100A8 primary antibody included.

产品信息

66853-1-PBS targets S100A8 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8517

Product name: Recombinant human S100A8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-93 aa of BC005928

Sequence: MLTELEKALNSIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIRKKGADVWFKELDINTDGAVNFQEFLILVIKMGVAAHKKSHEESHKE

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 S100 calcium binding protein A8
别名 CFAG, Calprotectin L1L subunit, Calgranulin-A, Calgranulin A, CAGA
计算分子量 93 aa, 11 kDa
GenBank蛋白编号BC005928
基因名称 S100A8
Gene ID (NCBI) 6279
RRIDAB_2882193
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05109
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine.

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