验证数据展示

WB analysis using 15792-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15792-1-AP (S100A8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse lung using 15792-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with S100A8 antibody included
IHC staining of mouse lung using 15792-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with S100A8 antibody included
IHC staining of rat spleen using 15792-1-AP
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with S100A8 antibody included
IHC staining of rat spleen using 15792-1-AP
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with S100A8 antibody included
IF Staining of mouse stomach using 15792-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using S100A8 antibody (15792-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse spleen using 15792-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using S100A8 antibody (15792-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse spleen using 15792-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using S100A8 antibody (15792-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse lung using 15792-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using S100A8 antibody (15792-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse lung using 15792-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using S100A8 antibody (15792-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP experiment of MCF-7 using 15792-1-AP
IP result of anti-S100A8 (IP:15792-1-AP, 4ug; Detection:15792-1-AP 1:600) with MCF-7 cells lysate 1400 ug.
IHC staining of rat spleen using 15792-1-AP
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with S100A8 antibody included
IHC staining of rat spleen using 15792-1-AP
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 15792-1-AP (S100A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with S100A8 antibody included
IF Staining of THP-1 using 15792-1-AP
Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using S100A8 antibody (15792-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

S100A8 Polyclonal antibody

S100A8 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 15792-1-AP

产品说明书

引用文献(87)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)

别名

CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, CFAG

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (87)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HL-60 cells, A549 cells, COLO 320 cells, THP-1 cells, MCF-7 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	mouse lung tissue, human stomach cancer tissue, rat spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse stomach tissue, mouse lung tissue, mouse spleen tissue
Positive IF/ICC detected in	THP-1 cells

Planning an IHC experiment? We recommend our IHCeasy S100A8 Ready-To-Use IHC Kit. S100A8 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 41 publications below
IHC	See 18 publications below
IF	See 21 publications below

产品信息

15792-1-AP targets S100A8 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag8500 Product name: Recombinant human S100A8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-93 aa of BC005928 Sequence: MLTELEKALNSIIDVYHKYSLIKGNFHAVYRDDLKKLLETECPQYIRKKGADVWFKELDINTDGAVNFQEFLILVIKMGVAAHKKSHEESHKE 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	S100 calcium binding protein A8
别名	CAGA, Calgranulin A, Calgranulin-A, Calprotectin L1L subunit, CFAG
计算分子量	93 aa, 11 kDa
观测分子量	11 kDa
GenBank蛋白编号	BC005928
基因名称	S100A8
Gene ID (NCBI)	6279
RRID	AB_10666315
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P05109
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. S100A8 may form homodimer or heterodimer with S100A9 ( PMID: 16216873).

实验方案

Product Specific Protocols
IF protocol for S100A8 antibody 15792-1-AP	Download protocol
IHC protocol for S100A8 antibody 15792-1-AP	Download protocol
IP protocol for S100A8 antibody 15792-1-AP	Download protocol
WB protocol for S100A8 antibody 15792-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IHC	Signal Transduct Target Ther The heterogeneous immune landscape between lung adenocarcinoma and squamous carcinoma revealed by single-cell RNA sequencing Authors - Chengdi Wang View Article
human	WB	Signal Transduct Target Ther Thromboxane receptor activation in dendritic cells mitigates sepsis by suppressing S100a8/a9-mediated neutrophil recruitment Authors - Ronglu Du View Article
human	IHC	Cancer Cell KRAS-IRF2 Axis Drives Immune Suppression and Immune Therapy Resistance in Colorectal Cancer. Authors - Wenting Liao View Article
human	IHC	Cell Metab QDPR deficiency drives immune suppression in pancreatic cancer Authors - Ji Liu View Article
human	IHC	Nat Biomed Eng Cancer biomarkers discovered using pan-cancer plasma proteomic profiling Authors - Lin Bai View Article
human	WB	Bone Res Human peripheral osteoclast-precursor-development patterns reveal the significance of RPS17-dependent ribosome synthesis to Ankylosing Spondylitis lesions. Authors - Dianshan Ke View Article

验证数据展示

WB analysis using 15792-1-AP

IHC staining of mouse lung using 15792-1-AP

IHC staining of mouse lung using 15792-1-AP

IHC staining of rat spleen using 15792-1-AP

IHC staining of rat spleen using 15792-1-AP

IF Staining of mouse stomach using 15792-1-AP

IF Staining of mouse spleen using 15792-1-AP

IF Staining of mouse spleen using 15792-1-AP

IF Staining of mouse lung using 15792-1-AP

IF Staining of mouse lung using 15792-1-AP

IP experiment of MCF-7 using 15792-1-AP

IHC staining of rat spleen using 15792-1-AP

IHC staining of rat spleen using 15792-1-AP

IF Staining of THP-1 using 15792-1-AP

S100A8 Polyclonal antibody

S100A8 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

The heterogeneous immune landscape between lung adenocarcinoma and squamous carcinoma revealed by single-cell RNA sequencing

Thromboxane receptor activation in dendritic cells mitigates sepsis by suppressing S100a8/a9-mediated neutrophil recruitment

KRAS-IRF2 Axis Drives Immune Suppression and Immune Therapy Resistance in Colorectal Cancer.

QDPR deficiency drives immune suppression in pancreatic cancer

Cancer biomarkers discovered using pan-cancer plasma proteomic profiling

Human peripheral osteoclast-precursor-development patterns reveal the significance of RPS17-dependent ribosome synthesis to Ankylosing Spondylitis lesions.

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